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Calreticulin-dependent recycling in the early secretory pathway mediates optimal peptide loading of MHC class I molecules

Calreticulin-dependent recycling in the early secretory pathway mediates optimal peptide loading of MHC class I molecules
Calreticulin-dependent recycling in the early secretory pathway mediates optimal peptide loading of MHC class I molecules
Calreticulin is a lectin chaperone of the endoplasmic reticulum (ER). In calreticulin-deficient cells, major histocompatibility complex (MHC) class I molecules travel to the cell surface in association with a sub-optimal peptide load. Here, we show that calreticulin exits the ER to accumulate in the ER–Golgi intermediate compartment (ERGIC) and the cis-Golgi, together with sub-optimally loaded class I molecules. Calreticulin that lacks its C-terminal KDEL retrieval sequence assembles with the peptide-loading complex but neither retrieves sub-optimally loaded class I molecules from the cis-Golgi to the ER, nor supports optimal peptide loading. Our study, to the best of our knowledge, demonstrates for the first time a functional role of intracellular transport in the optimal loading of MHC class I molecules with antigenic peptide.
0261-4189
3730-3744
Howe, Christopher
08a609ff-f7b9-412a-8e62-a35a40736bf6
Garstka, Malgorzata
96cdbefc-4080-424c-b1f0-fd851224824f
Al-Balushi, Mohammed
87fc0a02-58c6-4e57-899e-2ea26e308a92
Ghanem, Esther
772798c2-c5ec-4a8a-96a5-0c523d52c1a9
Antoniou, Antony N.
3d711a3b-f688-46f9-823e-5bb925d10ea8
Fritzsche, Susanne
b0bc2ab6-d954-468f-9075-772553cc155f
Jankevicius, Gytis
68aa8b80-dc65-4b52-8990-1a786dcd8e29
Kontouli, Nasia
16b29ba5-2378-432f-b5a2-6971a868408e
Schneeweiss, Clemens
ed8a31e7-1493-4b96-85cf-e3035fda6fc3
Williams, Anthony
973ff46f-46f1-4d7c-b27d-0f53221e4c44
Springer, Sebastian
e903d13d-e3ab-496d-a0ae-a92261d266d5
Elliott, Tim
16670fa8-c2f9-477a-91df-7c9e5b453e0e
Howe, Christopher
08a609ff-f7b9-412a-8e62-a35a40736bf6
Garstka, Malgorzata
96cdbefc-4080-424c-b1f0-fd851224824f
Al-Balushi, Mohammed
87fc0a02-58c6-4e57-899e-2ea26e308a92
Ghanem, Esther
772798c2-c5ec-4a8a-96a5-0c523d52c1a9
Antoniou, Antony N.
3d711a3b-f688-46f9-823e-5bb925d10ea8
Fritzsche, Susanne
b0bc2ab6-d954-468f-9075-772553cc155f
Jankevicius, Gytis
68aa8b80-dc65-4b52-8990-1a786dcd8e29
Kontouli, Nasia
16b29ba5-2378-432f-b5a2-6971a868408e
Schneeweiss, Clemens
ed8a31e7-1493-4b96-85cf-e3035fda6fc3
Williams, Anthony
973ff46f-46f1-4d7c-b27d-0f53221e4c44
Springer, Sebastian
e903d13d-e3ab-496d-a0ae-a92261d266d5
Elliott, Tim
16670fa8-c2f9-477a-91df-7c9e5b453e0e

Howe, Christopher, Garstka, Malgorzata, Al-Balushi, Mohammed, Ghanem, Esther, Antoniou, Antony N., Fritzsche, Susanne, Jankevicius, Gytis, Kontouli, Nasia, Schneeweiss, Clemens, Williams, Anthony, Springer, Sebastian and Elliott, Tim (2009) Calreticulin-dependent recycling in the early secretory pathway mediates optimal peptide loading of MHC class I molecules The EMBO Journal, 28, (23), pp. 3730-3744. (doi:10.1038/emboj.2009.296).

Record type: Article

Abstract

Calreticulin is a lectin chaperone of the endoplasmic reticulum (ER). In calreticulin-deficient cells, major histocompatibility complex (MHC) class I molecules travel to the cell surface in association with a sub-optimal peptide load. Here, we show that calreticulin exits the ER to accumulate in the ER–Golgi intermediate compartment (ERGIC) and the cis-Golgi, together with sub-optimally loaded class I molecules. Calreticulin that lacks its C-terminal KDEL retrieval sequence assembles with the peptide-loading complex but neither retrieves sub-optimally loaded class I molecules from the cis-Golgi to the ER, nor supports optimal peptide loading. Our study, to the best of our knowledge, demonstrates for the first time a functional role of intracellular transport in the optimal loading of MHC class I molecules with antigenic peptide.

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Published date: 2 December 2009
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 73282
URI: http://eprints.soton.ac.uk/id/eprint/73282
ISSN: 0261-4189
PURE UUID: 88dfae00-a16c-40ad-be9c-65726ab7dc8d
ORCID for Tim Elliott: ORCID iD orcid.org/0000-0003-1097-0222

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Date deposited: 04 Mar 2010
Last modified: 18 Jul 2017 23:50

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Contributors

Author: Christopher Howe
Author: Malgorzata Garstka
Author: Mohammed Al-Balushi
Author: Esther Ghanem
Author: Antony N. Antoniou
Author: Susanne Fritzsche
Author: Gytis Jankevicius
Author: Nasia Kontouli
Author: Clemens Schneeweiss
Author: Sebastian Springer
Author: Tim Elliott ORCID iD

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