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Comparison of L-serine uptake by human placental microvillous membrane vesicles and placental villous fragments

Comparison of L-serine uptake by human placental microvillous membrane vesicles and placental villous fragments
Comparison of L-serine uptake by human placental microvillous membrane vesicles and placental villous fragments
Both syncytiotrophoblast microvillous plasma membrane vesicles (MVM) and placental villous fragments are used to characterize the placental uptake of maternal substrate and to investigate changes in uptake associated with pathological conditions. However, the two techniques have not been directly compared. In this study uptake of 14C-l-serine was compared in placental villous fragments and in MVM prepared from the same placentas.

14C-l-serine uptake into MVM vesicles was mediated by System L and System A and smaller unidentified Na+-dependent and Na+-independent components. In villous fragments an unidentified Na+-dependent component mediated the majority of 14C-l-serine uptake followed by System A and System L. The unidentified Na+-independent component of l-serine uptake was not detected in villous fragments.

The ratio of System A activity to System L activity was similar in villous fragments and MVM vesicles. However, the unidentified Na+-dependent component in villous fragments was significantly higher than that in MVM vesicles. This indicates that the main differences in serine uptake mechanisms identified using the two techniques were not due to differences in System A and System L activity but to differences in the unidentified Na+-dependent component.

This study suggests that uptake of l-serine into MVM vesicles and villous fragments via Systems A and L is comparable, but that this is not true for all components of l-serine uptake
amino acid, transport, system a, system l
0143-4004
456-459
Brand, A.P.
1465c13a-5efc-4328-bbc2-5e61e9523f3c
Greenwood, S.L.
09a72af7-5c7f-47f0-a4ba-89887758d6e7
Bennett, E.J.
828b30bb-ac41-4741-9ea0-e4b8391d5c02
Godfrey, K.M.
0931701e-fe2c-44b5-8f0d-ec5c7477a6fd
Sibley, C.P.
6e4ffcd0-f4b5-41c4-8360-9d78b9e9156c
Hanson, M.A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Lewis, R.M.
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502
Brand, A.P.
1465c13a-5efc-4328-bbc2-5e61e9523f3c
Greenwood, S.L.
09a72af7-5c7f-47f0-a4ba-89887758d6e7
Bennett, E.J.
828b30bb-ac41-4741-9ea0-e4b8391d5c02
Godfrey, K.M.
0931701e-fe2c-44b5-8f0d-ec5c7477a6fd
Sibley, C.P.
6e4ffcd0-f4b5-41c4-8360-9d78b9e9156c
Hanson, M.A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Lewis, R.M.
caaeb97d-ea69-4f7b-8adb-5fa25e2d3502

Brand, A.P., Greenwood, S.L., Bennett, E.J., Godfrey, K.M., Sibley, C.P., Hanson, M.A. and Lewis, R.M. (2009) Comparison of L-serine uptake by human placental microvillous membrane vesicles and placental villous fragments. Placenta, 31 (5), 456-459. (doi:10.1016/j.placenta.2010.01.016).

Record type: Article

Abstract

Both syncytiotrophoblast microvillous plasma membrane vesicles (MVM) and placental villous fragments are used to characterize the placental uptake of maternal substrate and to investigate changes in uptake associated with pathological conditions. However, the two techniques have not been directly compared. In this study uptake of 14C-l-serine was compared in placental villous fragments and in MVM prepared from the same placentas.

14C-l-serine uptake into MVM vesicles was mediated by System L and System A and smaller unidentified Na+-dependent and Na+-independent components. In villous fragments an unidentified Na+-dependent component mediated the majority of 14C-l-serine uptake followed by System A and System L. The unidentified Na+-independent component of l-serine uptake was not detected in villous fragments.

The ratio of System A activity to System L activity was similar in villous fragments and MVM vesicles. However, the unidentified Na+-dependent component in villous fragments was significantly higher than that in MVM vesicles. This indicates that the main differences in serine uptake mechanisms identified using the two techniques were not due to differences in System A and System L activity but to differences in the unidentified Na+-dependent component.

This study suggests that uptake of l-serine into MVM vesicles and villous fragments via Systems A and L is comparable, but that this is not true for all components of l-serine uptake

Full text not available from this repository.

More information

Published date: 2009
Keywords: amino acid, transport, system a, system l

Identifiers

Local EPrints ID: 73299
URI: https://eprints.soton.ac.uk/id/eprint/73299
ISSN: 0143-4004
PURE UUID: 7e2d4c47-aa46-46af-9ed5-6746c0e18093
ORCID for K.M. Godfrey: ORCID iD orcid.org/0000-0002-4643-0618
ORCID for M.A. Hanson: ORCID iD orcid.org/0000-0002-6907-613X
ORCID for R.M. Lewis: ORCID iD orcid.org/0000-0003-4044-9104

Catalogue record

Date deposited: 04 Mar 2010
Last modified: 20 Jul 2019 01:22

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