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Molecular microbiological characterization of preterm neonatesat risk of bronchopulmonary dysplasia

Molecular microbiological characterization of preterm neonatesat risk of bronchopulmonary dysplasia
Molecular microbiological characterization of preterm neonatesat risk of bronchopulmonary dysplasia
The role of infection in bronchopulmonary dysplasia (BPD) is unknown. We present an observational study of 55 premature infants born weighing less than 1.3 kg within two level III neonatal intensive care units. Endotracheal aspirates (ETA) and nasogastric aspirates (NGA) were studied with denaturing gradient gel electrophoresis (DGGE) profiling to elucidate the total bacterial community, and species-specific PCR was used to detect the presence of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. DGGE identified bacterial species in 59% of NGA and ETA samples combined. A diverse range of species were identified including several implicated in preterm labor. Species-specific PCR identified M. hominis in 25% of NGA and 11% of ETA samples. Among the 48 infants surviving up to 36 wk-postconceptual age, ordinal logistic regression showed the odds ratio for BPD or death where Ureaplasma was present/absent as 4.80 (95% CI 1.15-20.13). After adjusting for number of days ventilated, this was reduced to 2.04 (0.41-10.25). These data demonstrate how the combined use of DGGE and species-specific PCR identifies a high exposure in utero and around the time of birth to bacteria that might be causally related to preterm delivery and subsequent lung injury.

0031-3998
412-418
Payne, Matthew S.
703a75af-45cf-4e43-bf55-232d42e2a468
Goss, Kevin C.W.
d9ab6445-af10-40da-8b27-2f8ecf03c3d8
Connett, Gary J.
55d5676c-90d8-46bf-a508-62eded276516
Kollamparambil, Tanoj
99119c8c-2785-48c3-a268-4b8ebfbbb226
Legg, Julian L.
094a1221-d175-48dd-a9bf-ee33d4ee5b91
Thwaites, Richard
9b36e044-fbff-46da-9c5c-61d7727a8304
Ashton, Mark
8503631c-04ae-4fe0-875e-9a8ab838c187
Puddy, Victoria
cec2ef1e-ee1f-410c-9720-76da4fb0530d
Peacock, Janet L.
1cb1242c-7606-4f8e-86d0-d3cd2ceff782
Bruce, Kenneth D.
09fa4b3f-2183-4e6e-933d-70a3c1b20b5d
Payne, Matthew S.
703a75af-45cf-4e43-bf55-232d42e2a468
Goss, Kevin C.W.
d9ab6445-af10-40da-8b27-2f8ecf03c3d8
Connett, Gary J.
55d5676c-90d8-46bf-a508-62eded276516
Kollamparambil, Tanoj
99119c8c-2785-48c3-a268-4b8ebfbbb226
Legg, Julian L.
094a1221-d175-48dd-a9bf-ee33d4ee5b91
Thwaites, Richard
9b36e044-fbff-46da-9c5c-61d7727a8304
Ashton, Mark
8503631c-04ae-4fe0-875e-9a8ab838c187
Puddy, Victoria
cec2ef1e-ee1f-410c-9720-76da4fb0530d
Peacock, Janet L.
1cb1242c-7606-4f8e-86d0-d3cd2ceff782
Bruce, Kenneth D.
09fa4b3f-2183-4e6e-933d-70a3c1b20b5d

Payne, Matthew S., Goss, Kevin C.W., Connett, Gary J., Kollamparambil, Tanoj, Legg, Julian L., Thwaites, Richard, Ashton, Mark, Puddy, Victoria, Peacock, Janet L. and Bruce, Kenneth D. (2010) Molecular microbiological characterization of preterm neonatesat risk of bronchopulmonary dysplasia. Pediatric Research, 67 (4), 412-418. (doi:10.1203/PDR.0b013e3181d026c3).

Record type: Article

Abstract

The role of infection in bronchopulmonary dysplasia (BPD) is unknown. We present an observational study of 55 premature infants born weighing less than 1.3 kg within two level III neonatal intensive care units. Endotracheal aspirates (ETA) and nasogastric aspirates (NGA) were studied with denaturing gradient gel electrophoresis (DGGE) profiling to elucidate the total bacterial community, and species-specific PCR was used to detect the presence of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. DGGE identified bacterial species in 59% of NGA and ETA samples combined. A diverse range of species were identified including several implicated in preterm labor. Species-specific PCR identified M. hominis in 25% of NGA and 11% of ETA samples. Among the 48 infants surviving up to 36 wk-postconceptual age, ordinal logistic regression showed the odds ratio for BPD or death where Ureaplasma was present/absent as 4.80 (95% CI 1.15-20.13). After adjusting for number of days ventilated, this was reduced to 2.04 (0.41-10.25). These data demonstrate how the combined use of DGGE and species-specific PCR identifies a high exposure in utero and around the time of birth to bacteria that might be causally related to preterm delivery and subsequent lung injury.

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More information

Submitted date: 2009
Accepted/In Press date: 2009
Published date: April 2010

Identifiers

Local EPrints ID: 73312
URI: http://eprints.soton.ac.uk/id/eprint/73312
ISSN: 0031-3998
PURE UUID: 6286cb84-c4f5-4d51-ac73-a874a8120dd8
ORCID for Gary J. Connett: ORCID iD orcid.org/0000-0003-1310-3239

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Date deposited: 10 Mar 2010
Last modified: 07 Aug 2019 00:22

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Contributors

Author: Matthew S. Payne
Author: Kevin C.W. Goss
Author: Gary J. Connett ORCID iD
Author: Tanoj Kollamparambil
Author: Julian L. Legg
Author: Richard Thwaites
Author: Mark Ashton
Author: Victoria Puddy
Author: Janet L. Peacock
Author: Kenneth D. Bruce

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