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Number of CD4+ cells and location of forkhead box protein P3-positive cells in diagnostic follicular lymphoma tissue microarrays correlates with outcome

Number of CD4+ cells and location of forkhead box protein P3-positive cells in diagnostic follicular lymphoma tissue microarrays correlates with outcome
Number of CD4+ cells and location of forkhead box protein P3-positive cells in diagnostic follicular lymphoma tissue microarrays correlates with outcome
Purpose
To examine the immune microenvironment in diagnostic follicular lymphoma (FL) biopsies and evaluate its prognostic significance.

Patients and Methods
Immunohistochemistry was used to study numbers and location of cells staining positive for immune cell markers CD4, CD7, CD8, CD25, CD68, forkhead box protein P3 (FOXP3), T-cell intracellular antigen-1, and Granzyme B in tissue microarrays of paraffin-embedded, diagnostic lymph node biopsies taken from 59 FL patients who lived less than 5 years (short-survival group; n = 34) and more than 15 years (long-survival group; n = 25).

Results
CD4 and FOXP3 expression were significantly different between the two groups. Samples from the long-survival group were more likely than those from the short-survival group to have CD4+ staining cells and to have FOXP3-positive cells in a perifollicular location.

Conclusion
This study has identified differences in immune cell composition of the diagnostic FL lymph node immune microenvironment and these have the potential for use as prognostic biomarkers in a routine histopathology setting.
1527-7755
5052-5059
Lee, Abigail M.
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Clear, Andrew J.
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Calaminici, Maria
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Davies, Andrew J.
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Jordan, Suzanne
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MacDougall, Finlay
49356af2-ed05-452a-a674-356cde64c2da
Matthews, Janet
baa9d6ab-0e42-4ac6-8a95-48ce92acc38b
Norton, Andrew J.
023ec40c-5e02-4922-b20d-0020a4e67743
Gribben, John G.
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Lister, T. Andrew
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Goff, Lindsey K.
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Lee, Abigail M.
ff9213d8-cf33-4dd3-927c-1208e3c0f43f
Clear, Andrew J.
d0c33dde-7eda-4664-af72-8d10eec561de
Calaminici, Maria
81ec2316-a0dc-49e7-a5db-0c7bdd525eb3
Davies, Andrew J.
0fe6a40a-10d1-4ade-a7e6-d1dceb2470af
Jordan, Suzanne
0797f792-c6dd-4fca-ae54-770cefaf59da
MacDougall, Finlay
49356af2-ed05-452a-a674-356cde64c2da
Matthews, Janet
baa9d6ab-0e42-4ac6-8a95-48ce92acc38b
Norton, Andrew J.
023ec40c-5e02-4922-b20d-0020a4e67743
Gribben, John G.
8ae0a436-65bb-4397-b09f-5eac89bf08ef
Lister, T. Andrew
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Goff, Lindsey K.
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Lee, Abigail M., Clear, Andrew J., Calaminici, Maria, Davies, Andrew J., Jordan, Suzanne, MacDougall, Finlay, Matthews, Janet, Norton, Andrew J., Gribben, John G., Lister, T. Andrew and Goff, Lindsey K. (2006) Number of CD4+ cells and location of forkhead box protein P3-positive cells in diagnostic follicular lymphoma tissue microarrays correlates with outcome. Journal of Clinical Oncology, 24 (31), 5052-5059. (doi:10.1200/JCO.2006.06.4642).

Record type: Article

Abstract

Purpose
To examine the immune microenvironment in diagnostic follicular lymphoma (FL) biopsies and evaluate its prognostic significance.

Patients and Methods
Immunohistochemistry was used to study numbers and location of cells staining positive for immune cell markers CD4, CD7, CD8, CD25, CD68, forkhead box protein P3 (FOXP3), T-cell intracellular antigen-1, and Granzyme B in tissue microarrays of paraffin-embedded, diagnostic lymph node biopsies taken from 59 FL patients who lived less than 5 years (short-survival group; n = 34) and more than 15 years (long-survival group; n = 25).

Results
CD4 and FOXP3 expression were significantly different between the two groups. Samples from the long-survival group were more likely than those from the short-survival group to have CD4+ staining cells and to have FOXP3-positive cells in a perifollicular location.

Conclusion
This study has identified differences in immune cell composition of the diagnostic FL lymph node immune microenvironment and these have the potential for use as prognostic biomarkers in a routine histopathology setting.

Full text not available from this repository.

More information

Published date: 1 November 2006

Identifiers

Local EPrints ID: 73328
URI: http://eprints.soton.ac.uk/id/eprint/73328
ISSN: 1527-7755
PURE UUID: 072da5de-f653-44d3-a19a-fb93d5b00ff1
ORCID for Andrew J. Davies: ORCID iD orcid.org/0000-0002-7517-6938

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Date deposited: 11 Mar 2010
Last modified: 03 Feb 2020 01:30

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Contributors

Author: Abigail M. Lee
Author: Andrew J. Clear
Author: Maria Calaminici
Author: Suzanne Jordan
Author: Finlay MacDougall
Author: Janet Matthews
Author: Andrew J. Norton
Author: John G. Gribben
Author: T. Andrew Lister
Author: Lindsey K. Goff

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