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The relative role of peripheral blood and bone marrow for monitoring molecular evidence of disease in follicular lymphoma by quantitative real-time polymerase chain reaction

The relative role of peripheral blood and bone marrow for monitoring molecular evidence of disease in follicular lymphoma by quantitative real-time polymerase chain reaction
The relative role of peripheral blood and bone marrow for monitoring molecular evidence of disease in follicular lymphoma by quantitative real-time polymerase chain reaction
Summary. Peripheral blood (PB) and bone marrow (BM) are used interchangeably for t(14;18) (IgH/BCL-2) molecular monitoring in follicular lymphoma (FL) and detection of rearrangement after treatment has been correlated to increased risk of relapse. To determine the relative value of each tissue, MBR t(14;18) was quantified by real-time polymerase chain reaction in 52 simultaneous paired PB and BM samples from 38 FL patients. In total, 79% of sample pairs taken in remission (n = 19) or when no morphological disease was evident in the BM (n = 29) had t(14;18) copy number within one log difference and the median difference was small. These findings suggest that, in remission, PB may be adequately monitored. In general, however, higher copy number was detected in BM than in the corresponding PB sample.
t(14, 18), follicular lymphoma, real-time pcr, monitoring, quantification
0007-1048
563-566
Summers, Karin E.
84d2701e-37d3-4ce8-99c9-ff7dee4e4673
Davies, Andrew J.
0fe6a40a-10d1-4ade-a7e6-d1dceb2470af
Matthews, Janet
baa9d6ab-0e42-4ac6-8a95-48ce92acc38b
Jenner, Michael J.
78c95aa3-2157-4a67-8ebb-d65a59892d17
Cornelius, Victoria
b75c21d7-2c25-495c-9107-e39453a72bdd
Amess, John A.
4f855dc4-7a16-4035-b020-9ba48beee3d0
Norton, Andrew J.
023ec40c-5e02-4922-b20d-0020a4e67743
Rohatiner, Ama Z. S.
c898ebce-6863-4a81-a4fb-694e673284f8
Fitzgibbon, Jude
6afae538-ec1c-41a0-af2a-21b98dfbb356
Lister, T. Andrew
6b42a014-ee50-4017-bbb8-16aa71b74018
Goff, Lindsey K.
980df9b7-ea16-489f-b3f6-01c7eb249bf7
Summers, Karin E.
84d2701e-37d3-4ce8-99c9-ff7dee4e4673
Davies, Andrew J.
0fe6a40a-10d1-4ade-a7e6-d1dceb2470af
Matthews, Janet
baa9d6ab-0e42-4ac6-8a95-48ce92acc38b
Jenner, Michael J.
78c95aa3-2157-4a67-8ebb-d65a59892d17
Cornelius, Victoria
b75c21d7-2c25-495c-9107-e39453a72bdd
Amess, John A.
4f855dc4-7a16-4035-b020-9ba48beee3d0
Norton, Andrew J.
023ec40c-5e02-4922-b20d-0020a4e67743
Rohatiner, Ama Z. S.
c898ebce-6863-4a81-a4fb-694e673284f8
Fitzgibbon, Jude
6afae538-ec1c-41a0-af2a-21b98dfbb356
Lister, T. Andrew
6b42a014-ee50-4017-bbb8-16aa71b74018
Goff, Lindsey K.
980df9b7-ea16-489f-b3f6-01c7eb249bf7

Summers, Karin E., Davies, Andrew J., Matthews, Janet, Jenner, Michael J., Cornelius, Victoria, Amess, John A., Norton, Andrew J., Rohatiner, Ama Z. S., Fitzgibbon, Jude, Lister, T. Andrew and Goff, Lindsey K. (2002) The relative role of peripheral blood and bone marrow for monitoring molecular evidence of disease in follicular lymphoma by quantitative real-time polymerase chain reaction. British Journal of Haematology, 118 (2), 563-566. (doi:10.1046/j.1365-2141.2002.03641.x).

Record type: Article

Abstract

Summary. Peripheral blood (PB) and bone marrow (BM) are used interchangeably for t(14;18) (IgH/BCL-2) molecular monitoring in follicular lymphoma (FL) and detection of rearrangement after treatment has been correlated to increased risk of relapse. To determine the relative value of each tissue, MBR t(14;18) was quantified by real-time polymerase chain reaction in 52 simultaneous paired PB and BM samples from 38 FL patients. In total, 79% of sample pairs taken in remission (n = 19) or when no morphological disease was evident in the BM (n = 29) had t(14;18) copy number within one log difference and the median difference was small. These findings suggest that, in remission, PB may be adequately monitored. In general, however, higher copy number was detected in BM than in the corresponding PB sample.

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Published date: August 2002
Keywords: t(14, 18), follicular lymphoma, real-time pcr, monitoring, quantification
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 73363
URI: http://eprints.soton.ac.uk/id/eprint/73363
ISSN: 0007-1048
PURE UUID: 09b908d6-48f8-4fa9-ade2-90b862c2c3e1
ORCID for Andrew J. Davies: ORCID iD orcid.org/0000-0002-7517-6938

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Date deposited: 09 Mar 2010
Last modified: 03 Feb 2020 01:30

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Contributors

Author: Karin E. Summers
Author: Janet Matthews
Author: Michael J. Jenner
Author: Victoria Cornelius
Author: John A. Amess
Author: Andrew J. Norton
Author: Ama Z. S. Rohatiner
Author: Jude Fitzgibbon
Author: T. Andrew Lister
Author: Lindsey K. Goff

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