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Absence of tapasin alters immunodominance against a lymphocytic choriomeningitis virus polytope

Record type: Article

Tapasin edits the peptide repertoire presented to CD8+ T cells by favoring loading of slow off-rate peptides on MHC I molecules. To investigate the role of tapasin on T cell immunodominance we used poxvirus viral vectors expressing a polytope of lymphocytic choriomeningitis virus epitopes with different off-rates. In tapasin-deficient mice, responses to subdominant fast off-rate peptides were clearly favored. This alteration of the CD8+ T cell hierarchy was a consequence of tapasin editing and not a consequence of the alteration of the T cell repertoire in tapasin-deficient mice, because bone marrow chimeric mice (wild-type recipients reconstituted with tapasin knockout bone marrow) showed the same hierarchy as the tapasin knockout mice. Tapasin editing is therefore a contributing factor to the phenomenon of immunodominance. Although tapasin knockout cells have low MHC I surface expression, Ag presentation was efficient and resulted in strong T cell responses involving T cells with increased functional avidity. Therefore, in this model, tapasin-deficient mice do not have a reduced but rather have an altered immune response.

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Citation

Boulanger, Denise S.M., Oliveira, Roberta, Ayres, Lisa, Prior, Stephen H., James, Edward, Williams, Anthony P. and Elliott, Tim (2010) Absence of tapasin alters immunodominance against a lymphocytic choriomeningitis virus polytope Journal of Immunology, 184, (1), pp. 73-83. (doi:10.4049/jimmunol.0803489).

More information

Accepted/In Press date: 30 November 2009
Published date: 1 January 2010
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 73379
URI: http://eprints.soton.ac.uk/id/eprint/73379
ISSN: 0022-1767
PURE UUID: 57e16a63-61b6-4045-9c69-f82922182f3d
ORCID for Tim Elliott: ORCID iD orcid.org/0000-0003-1097-0222

Catalogue record

Date deposited: 05 Mar 2010
Last modified: 18 Jul 2017 23:50

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Contributors

Author: Denise S.M. Boulanger
Author: Roberta Oliveira
Author: Lisa Ayres
Author: Stephen H. Prior
Author: Edward James
Author: Tim Elliott ORCID iD

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