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Determining the absolute abundance of dinoflagellate cysts in recent marine sediments: The Lycopodium marker-grain method put to the test

Determining the absolute abundance of dinoflagellate cysts in recent marine sediments: The Lycopodium marker-grain method put to the test
Determining the absolute abundance of dinoflagellate cysts in recent marine sediments: The Lycopodium marker-grain method put to the test
Absolute abundances (concentrations) of dinoflagellate cysts are often determined through the addition of Lycopodium clavatum marker-grains as a spike to a sample before palynological processing. An inter-laboratory calibration exercise was set up in order to test the comparability of results obtained in different laboratories, each using its own preparation method. Each of the 23 laboratories received the same amount of homogenized splits of four Quaternary sediment samples. The samples originate from different localities and consisted of a variety of lithologies. Dinoflagellate cysts were extracted and counted, and relative and absolute abundances were calculated. The relative abundances proved to be fairly reproducible, notwithstanding a need for taxonomic calibration. By contrast, excessive loss of Lycopodium spores during sample preparation resulted in non-reproducibility of absolute abundances. Use of oxidation, KOH, warm acids, acetolysis, mesh sizes larger than 15 µm and long ultrasonication (> 1 min) must be avoided to determine reproducible absolute abundances. The results of this work therefore indicate that the dinoflagellate cyst worker should make a choice between using the proposed standard method which circumvents critical steps, adding Lycopodium tablets at the end of the preparation and using an alternative method.

0034-6667
238-252
Mertens, Kenneth Neil
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Verhoeven, Koen
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Verleye, Thomas
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Louwye, Stephen
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Amorim, Ana
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Ribeiro, Sofia
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Harding, Ian C.
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De Schepper, Stijn
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González, Catalina
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De Vernal, Anne
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Henry, Maryse
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Radi, Taoufik
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Poulsen, Niels E.
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Heilmann-Clausen, Claus
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Londeix, Laurent
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Prasad, Vandana
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Pospelova, Vera
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Kyffin Hughes, Jane E.
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Rochon, André
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Sangiorgi, Francesca
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Welters, Natasja
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Sinclair, Natalie
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Soliman, Ali
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Van Nieuwenhove, Nicolas
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Vink, Annemiek
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Young, Martin
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Mertens, Kenneth Neil
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Verhoeven, Koen
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Verleye, Thomas
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Louwye, Stephen
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Amorim, Ana
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Ribeiro, Sofia
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Deaf, Amr S.
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Harding, Ian C.
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De Schepper, Stijn
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Londeix, Laurent
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Turon, Jean-Louis
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Matthiessen, Jens
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McCarthy, Francine M.G.
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Prasad, Vandana
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Pospelova, Vera
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Kyffin Hughes, Jane E.
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Riding, James B.
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Rochon, André
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Sangiorgi, Francesca
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Sinclair, Natalie
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Soliman, Ali
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Van Nieuwenhove, Nicolas
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Vink, Annemiek
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Young, Martin
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Mertens, Kenneth Neil, Verhoeven, Koen, Verleye, Thomas, Louwye, Stephen, Amorim, Ana, Ribeiro, Sofia, Deaf, Amr S., Harding, Ian C., De Schepper, Stijn, González, Catalina, Kodrans-Nsiah, Monika, De Vernal, Anne, Henry, Maryse, Radi, Taoufik, Dybkjaer, Karen, Poulsen, Niels E., Feist-Burkhardt, Susanne, Chitolie, Jonah, Heilmann-Clausen, Claus, Londeix, Laurent, Turon, Jean-Louis, Marret, Fabienne, Matthiessen, Jens, McCarthy, Francine M.G., Prasad, Vandana, Pospelova, Vera, Kyffin Hughes, Jane E., Riding, James B., Rochon, André, Sangiorgi, Francesca, Welters, Natasja, Sinclair, Natalie, Thun, Christian, Soliman, Ali, Van Nieuwenhove, Nicolas, Vink, Annemiek and Young, Martin (2009) Determining the absolute abundance of dinoflagellate cysts in recent marine sediments: The Lycopodium marker-grain method put to the test. Review of Palaeobotany and Palynology, 157 (3-4), 238-252. (doi:10.1016/j.revpalbo.2009.05.004).

Record type: Article

Abstract

Absolute abundances (concentrations) of dinoflagellate cysts are often determined through the addition of Lycopodium clavatum marker-grains as a spike to a sample before palynological processing. An inter-laboratory calibration exercise was set up in order to test the comparability of results obtained in different laboratories, each using its own preparation method. Each of the 23 laboratories received the same amount of homogenized splits of four Quaternary sediment samples. The samples originate from different localities and consisted of a variety of lithologies. Dinoflagellate cysts were extracted and counted, and relative and absolute abundances were calculated. The relative abundances proved to be fairly reproducible, notwithstanding a need for taxonomic calibration. By contrast, excessive loss of Lycopodium spores during sample preparation resulted in non-reproducibility of absolute abundances. Use of oxidation, KOH, warm acids, acetolysis, mesh sizes larger than 15 µm and long ultrasonication (> 1 min) must be avoided to determine reproducible absolute abundances. The results of this work therefore indicate that the dinoflagellate cyst worker should make a choice between using the proposed standard method which circumvents critical steps, adding Lycopodium tablets at the end of the preparation and using an alternative method.

Full text not available from this repository.

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Published date: November 2009

Identifiers

Local EPrints ID: 73465
URI: http://eprints.soton.ac.uk/id/eprint/73465
ISSN: 0034-6667
PURE UUID: f6721599-cb46-4150-ad04-7055ca3149bf
ORCID for Ian C. Harding: ORCID iD orcid.org/0000-0003-4281-0581

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Date deposited: 05 Mar 2010
Last modified: 22 Oct 2019 00:56

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Contributors

Author: Kenneth Neil Mertens
Author: Koen Verhoeven
Author: Thomas Verleye
Author: Stephen Louwye
Author: Ana Amorim
Author: Sofia Ribeiro
Author: Amr S. Deaf
Author: Ian C. Harding ORCID iD
Author: Stijn De Schepper
Author: Catalina González
Author: Monika Kodrans-Nsiah
Author: Anne De Vernal
Author: Maryse Henry
Author: Taoufik Radi
Author: Karen Dybkjaer
Author: Niels E. Poulsen
Author: Susanne Feist-Burkhardt
Author: Jonah Chitolie
Author: Claus Heilmann-Clausen
Author: Laurent Londeix
Author: Jean-Louis Turon
Author: Fabienne Marret
Author: Jens Matthiessen
Author: Francine M.G. McCarthy
Author: Vandana Prasad
Author: Vera Pospelova
Author: Jane E. Kyffin Hughes
Author: James B. Riding
Author: André Rochon
Author: Francesca Sangiorgi
Author: Natasja Welters
Author: Natalie Sinclair
Author: Christian Thun
Author: Ali Soliman
Author: Nicolas Van Nieuwenhove
Author: Annemiek Vink
Author: Martin Young

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