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Validation and comparison of two multiplex technologies, Luminex® and Mesoscale Discovery, for human cytokine profiling

Validation and comparison of two multiplex technologies, Luminex® and Mesoscale Discovery, for human cytokine profiling
Validation and comparison of two multiplex technologies, Luminex® and Mesoscale Discovery, for human cytokine profiling
Biomarker research has rapidly expanded over recent years aided by the progressive development of research tools, in particular the different multiplex technologies allowing simultaneous measurement of multiple analytes. It is foreseeable that such technology will have an integral role in clinical studies for establishing biomarker profiles of disease status, but validation of the tools is essential to confirm the reliability of their application. More comparable studies between multiplex platforms are required to enable users to determine which of these are best for a particular clinical study, as different platforms will have varying levels of performance for the validation parameters. Comparison of two multiplex platforms, the Luminex® and the Mesoscale Discovery, has been performed to determine their performance for the validation parameters of sensitivity, precision and accuracy for the cytokines IL-2, IL-4, IL-8, IL-10, IL-12, IFN? and TNF?. When measuring high concentrations both platforms show good accuracy (within +/? 25% recovery) with all cytokines except IL-12 for the MSD. At low concentrations, +/? 25% recovery was seen with all cytokines except IL-2 and IL-8 for the Luminex and IL-2 and IL-12 for the MSD. Although quantitative differences are found, relative differences are comparable, and consequently both platforms have been shown to be suitable for analyzing trends in multiple cytokine profiles, with the Luminex having better precision and the Mesoscale Discovery having greater sensitivity.
multiplex, cytokine, validation
0022-1759
55-64
Chowdhury, Ferdousi
0af499d4-17c5-40cf-9426-0d509ab82595
Williams, Anthiny
973ff46f-46f1-4d7c-b27d-0f53221e4c44
Johnson, Peter
3f6068ce-171e-4c2c-aca9-dc9b6a37413f
Chowdhury, Ferdousi
0af499d4-17c5-40cf-9426-0d509ab82595
Williams, Anthiny
973ff46f-46f1-4d7c-b27d-0f53221e4c44
Johnson, Peter
3f6068ce-171e-4c2c-aca9-dc9b6a37413f

Chowdhury, Ferdousi, Williams, Anthiny and Johnson, Peter (2009) Validation and comparison of two multiplex technologies, Luminex® and Mesoscale Discovery, for human cytokine profiling. Journal of Immunological Methods, 340 (1), 55-64. (doi:10.1016/j.jim.2008.10.002).

Record type: Article

Abstract

Biomarker research has rapidly expanded over recent years aided by the progressive development of research tools, in particular the different multiplex technologies allowing simultaneous measurement of multiple analytes. It is foreseeable that such technology will have an integral role in clinical studies for establishing biomarker profiles of disease status, but validation of the tools is essential to confirm the reliability of their application. More comparable studies between multiplex platforms are required to enable users to determine which of these are best for a particular clinical study, as different platforms will have varying levels of performance for the validation parameters. Comparison of two multiplex platforms, the Luminex® and the Mesoscale Discovery, has been performed to determine their performance for the validation parameters of sensitivity, precision and accuracy for the cytokines IL-2, IL-4, IL-8, IL-10, IL-12, IFN? and TNF?. When measuring high concentrations both platforms show good accuracy (within +/? 25% recovery) with all cytokines except IL-12 for the MSD. At low concentrations, +/? 25% recovery was seen with all cytokines except IL-2 and IL-8 for the Luminex and IL-2 and IL-12 for the MSD. Although quantitative differences are found, relative differences are comparable, and consequently both platforms have been shown to be suitable for analyzing trends in multiple cytokine profiles, with the Luminex having better precision and the Mesoscale Discovery having greater sensitivity.

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More information

Published date: 2009
Keywords: multiplex, cytokine, validation

Identifiers

Local EPrints ID: 73503
URI: https://eprints.soton.ac.uk/id/eprint/73503
ISSN: 0022-1759
PURE UUID: d98e1b83-5d2d-422c-93c2-715002451f13
ORCID for Peter Johnson: ORCID iD orcid.org/0000-0003-2306-4974

Catalogue record

Date deposited: 08 Mar 2010
Last modified: 05 Nov 2019 01:58

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