The University of Southampton
University of Southampton Institutional Repository

Transcriptional profiling of rats subjected to gestational undernourishment: implications for the developmental variations in metabolic traits

Morris, Tiffany J., Vickers, Mark, Gluckman, Peter, Gilmour, Stewart and Affara, Nabeel (2009) Transcriptional profiling of rats subjected to gestational undernourishment: implications for the developmental variations in metabolic traits PLoS ONE, 4, (9(e7271)), pp. 1-12. (doi:10.1371/journal.pone.0007271).

Record type: Article

Abstract

A link has been established between prenatal nutrition and the development of metabolic and cardiovascular diseases later in life, a process referred to as developmental programming. It has been suggested that the trajectory of development is shifted by alterations in the maternal nutritional state leading to changes in developmental plasticity, in part underpinned by epigenetic changes in gene regulation. However, to date, only candidate gene approaches have been used to assess expression and molecular changes in the offspring of maternally undernourished animals. Furthermore, most work has focused on animals at an age where the programmed phenotype is already manifest and little is known about changes in gene expression in the offspring prior to development of obesity and related metabolic disorders. Gene expression profiles of liver, retroperitoneal white adipose fat, and biceps femoris skeletal muscle tissue from young adult male rats (55 days old) in which nutritional status had been manipulated in utero by maternal undernutrition (UN) were compared to the profiles of offspring of ad libitum fed mothers serving as the control group (AD) (8 offspring/group). The expression profiles were determined using the Illumina RatRef-12 BeadChip. No significant changes in expression were identified for skeletal muscle or white adipose tissue. However, studies of liver tissue showed 249 differentially expressed genes (143 up regulated, 106 down regulated). Although the animals at day 55 have yet to develop obesity they already show biochemical abnormalities and by day 110 express a phenotype characterized by increased adiposity and altered insulin sensitivity. An analysis of pathways affected suggests that intrauterine programming of UN animals to favor fat as an energy source results in mitochondrial dysfunction which initially affects the postnatal hepatic function and subsequently, via the resultant metabolic changes in other organs leads to the evolution of a phenotype similar to that of the metabolic syndrome.

Other fetchObject.action_uri=info_doi%2F10.1371%2Fjournal.pone.0007271&representation=PDF - Version of Record
Available under License Other.
Download (264kB)

More information

Published date: 29 September 2009

Identifiers

Local EPrints ID: 73560
URI: http://eprints.soton.ac.uk/id/eprint/73560
ISSN: 1932-6203
PURE UUID: f110e835-7ae9-4df8-a3ab-7afdcaf51349

Catalogue record

Date deposited: 09 Mar 2010
Last modified: 18 Jul 2017 23:49

Export record

Altmetrics

Contributors

Author: Tiffany J. Morris
Author: Mark Vickers
Author: Peter Gluckman
Author: Stewart Gilmour
Author: Nabeel Affara

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×