The University of Southampton
University of Southampton Institutional Repository

Sonication of activated sludge flocs and the recovery of their bacteria on solid media

Banks, C.J. and Walker, I. (1977) Sonication of activated sludge flocs and the recovery of their bacteria on solid media Microbiology, 98, pp. 363-368. (doi:10.1099/00221287-98-2-363).

Record type: Article

Abstract

To enumerate the bacteria present in activated sludge it is necessary to release them undamaged from the flocs. Samples of diluted activated sludge were sonicated for periods ranging from 20 to 240 s at five different intensities. During sonication, floc disruption was followed by measuring the absorbance of treated samples at 400 and 610 nm, energy input by recording changes in temperature and the release of viable organisms by plating techniques.

The release of bacteria depended on the intensity and duration of sonication. Most viable bacteria were recovered after 80 to IOO s at a power output of 26 J s-l. Patterns of recovery of viable bacteria from 10 different activated sludges were of two types. Comparable recovery of bacteria from identical samples showed that two instruments could be successfully compared using physical criteria only. After sonication the number of bacteria recovered depended on the isolation medium. More bacteria were recovered from Casitone glycerol yeast extract agar than from Tryptone glucose beef and yeast extract vitamins agar (TGEVA) for five out of six sludges, and more were recovered from minimal pyruvate than from TGEVA for three out of six sludges.

Full text not available from this repository.

More information

Published date: 9 June 1977

Identifiers

Local EPrints ID: 74931
URI: http://eprints.soton.ac.uk/id/eprint/74931
ISSN: 1350-0872
PURE UUID: daa1c02a-3a27-49e1-af11-092951a78ef8

Catalogue record

Date deposited: 11 Mar 2010
Last modified: 18 Jul 2017 23:44

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×