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The effects of the most important phenolic constituents of olive mill wastewater on batch anaerobic methanogenesis

The effects of the most important phenolic constituents of olive mill wastewater on batch anaerobic methanogenesis
The effects of the most important phenolic constituents of olive mill wastewater on batch anaerobic methanogenesis
The most important phenolic constituents of Olive Mill Wastewater (OMW), i.e. tyrosol, oleuropein and caffeic, p-hydroxybenzoic and protocatechuic acids, were added at various concentrations to aliquots of domestic anaerobic sludge in Hungate serum bottles and these were incubated at 35°C. The concentration of methane in the headspace gas was monitored to determine if the phenolics were fermented to methane or if they inhibited the anaerobic process. Only tyrosol and p-hydroxybenzoic acid were fermented to methane. At 600 mg l-1 (but not at 400 mgl-1) oleuropein reduced the rate and the amount of methane produced. The ortho-diphenols (caffeic and protocatechuic acids) were inhibitory at 1000 mg l-1 but not at 500 mg l-1. In cultures supplemented with acetic and propionic acids (VFA), and in unsupplemented cultures, tyrosol at concentrations up to 600 mg l-1 was fermented to methane. Between 900 and 1300 mg l-1 tyrosol methane production was neither enhanced nor inhibited relative to control cultures containing no tyrosol. Inhibition of methane production was evident when tyrosol was present at ? 2000 mg l-1. In similar experiments with p-hydroxybenzoic acid enhanced methane production was observed at concentrations of ? 500 mg l-1; no enhancement or inhibition was observed at 700 mg l-1; and inhibition was noted when p-hydroxybenzoic acid was present at ? 1000 mg l-1.
phenolic compounds, methane, anaerobic digestion, inhibition, olive mill wastewater
0959-3330
167-174
Banks, C.J.
5c6c8c4b-5b25-4e37-9058-50fa8d2e926f
Borja, R.
ed513484-04ff-4424-ab79-dc715ca63146
Maestro, D.R.
4efdd3d4-2abe-4f31-881c-f158c572ca7d
Alba, J.
0d5b56ef-a2d6-44dd-8564-320a5482108e
Banks, C.J.
5c6c8c4b-5b25-4e37-9058-50fa8d2e926f
Borja, R.
ed513484-04ff-4424-ab79-dc715ca63146
Maestro, D.R.
4efdd3d4-2abe-4f31-881c-f158c572ca7d
Alba, J.
0d5b56ef-a2d6-44dd-8564-320a5482108e

Banks, C.J., Borja, R., Maestro, D.R. and Alba, J. (1996) The effects of the most important phenolic constituents of olive mill wastewater on batch anaerobic methanogenesis. Environmental Technology, 17 (2), 167-174. (doi:10.1080/09593331708616373).

Record type: Article

Abstract

The most important phenolic constituents of Olive Mill Wastewater (OMW), i.e. tyrosol, oleuropein and caffeic, p-hydroxybenzoic and protocatechuic acids, were added at various concentrations to aliquots of domestic anaerobic sludge in Hungate serum bottles and these were incubated at 35°C. The concentration of methane in the headspace gas was monitored to determine if the phenolics were fermented to methane or if they inhibited the anaerobic process. Only tyrosol and p-hydroxybenzoic acid were fermented to methane. At 600 mg l-1 (but not at 400 mgl-1) oleuropein reduced the rate and the amount of methane produced. The ortho-diphenols (caffeic and protocatechuic acids) were inhibitory at 1000 mg l-1 but not at 500 mg l-1. In cultures supplemented with acetic and propionic acids (VFA), and in unsupplemented cultures, tyrosol at concentrations up to 600 mg l-1 was fermented to methane. Between 900 and 1300 mg l-1 tyrosol methane production was neither enhanced nor inhibited relative to control cultures containing no tyrosol. Inhibition of methane production was evident when tyrosol was present at ? 2000 mg l-1. In similar experiments with p-hydroxybenzoic acid enhanced methane production was observed at concentrations of ? 500 mg l-1; no enhancement or inhibition was observed at 700 mg l-1; and inhibition was noted when p-hydroxybenzoic acid was present at ? 1000 mg l-1.

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More information

Published date: 1996
Additional Information: Banks C. J., Borja R, Maestro D R, Alba J, (1996). "The effects of the most important phenolic constituents of olive mill wastewater on batch anaerobic methanogenesis." Environmental Technology, 17(2), 167-174
Keywords: phenolic compounds, methane, anaerobic digestion, inhibition, olive mill wastewater

Identifiers

Local EPrints ID: 75304
URI: https://eprints.soton.ac.uk/id/eprint/75304
ISSN: 0959-3330
PURE UUID: 0f7f81e9-5990-43e7-b80d-b41117ba4723

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Date deposited: 11 Mar 2010
Last modified: 17 Jul 2019 00:15

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