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Protoplast isolation, culture and plant regeneration in the winged bean, Psophocarpus tetragonolobus (L) D.C.

Protoplast isolation, culture and plant regeneration in the winged bean, Psophocarpus tetragonolobus (L) D.C.
Protoplast isolation, culture and plant regeneration in the winged bean, Psophocarpus tetragonolobus (L) D.C.
Protoplasts have been isolated from winged bean (Psophocarpus tetragonolobus) suspension culture cells using a 2-mercaptoethanol supplemented enzyme solution. These protoplasts can be induced to divide and by continued proliferation give rise to callus colonies. Shoots have been regenerated from this callus. These shoots can be rooted and subsequently the plantlets develop to maturity.

isolation, culture, protoplasts, plant regeneration, winged bean
0168-9452
61-68
Wilson, V.M.
5db93f32-2925-4694-a22c-24431b144697
Haq, N.
d59a37ec-54c6-4267-be57-de498ae37c0b
Evans, P.K.
376a8687-6944-4dc3-aaa0-d3a040328f30
Wilson, V.M.
5db93f32-2925-4694-a22c-24431b144697
Haq, N.
d59a37ec-54c6-4267-be57-de498ae37c0b
Evans, P.K.
376a8687-6944-4dc3-aaa0-d3a040328f30

Wilson, V.M., Haq, N. and Evans, P.K. (1985) Protoplast isolation, culture and plant regeneration in the winged bean, Psophocarpus tetragonolobus (L) D.C. Plant Science, 41 (1), 61-68. (doi:10.1016/0168-9452(85)90066-4).

Record type: Article

Abstract

Protoplasts have been isolated from winged bean (Psophocarpus tetragonolobus) suspension culture cells using a 2-mercaptoethanol supplemented enzyme solution. These protoplasts can be induced to divide and by continued proliferation give rise to callus colonies. Shoots have been regenerated from this callus. These shoots can be rooted and subsequently the plantlets develop to maturity.

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More information

Published date: 3 October 1985
Keywords: isolation, culture, protoplasts, plant regeneration, winged bean

Identifiers

Local EPrints ID: 75722
URI: http://eprints.soton.ac.uk/id/eprint/75722
DOI: doi:10.1016/0168-9452(85)90066-4
ISSN: 0168-9452
PURE UUID: 7f7b90d2-1314-4387-9468-da9423b90804

Catalogue record

Date deposited: 11 Mar 2010
Last modified: 13 Mar 2024 23:01

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Contributors

Author: V.M. Wilson
Author: N. Haq
Author: P.K. Evans

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