Protoplast isolation, culture and plant regeneration in the winged bean, Psophocarpus tetragonolobus (L) D.C.


Wilson, V.M., Haq, N. and Evans, P.K. (1985) Protoplast isolation, culture and plant regeneration in the winged bean, Psophocarpus tetragonolobus (L) D.C. Plant Science, 41, (1), pp. 61-68. (doi:10.1016/0168-9452(85)90066-4).

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Description/Abstract

Protoplasts have been isolated from winged bean (Psophocarpus tetragonolobus) suspension culture cells using a 2-mercaptoethanol supplemented enzyme solution. These protoplasts can be induced to divide and by continued proliferation give rise to callus colonies. Shoots have been regenerated from this callus. These shoots can be rooted and subsequently the plantlets develop to maturity.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/0168-9452(85)90066-4
ISSNs: 0168-9452 (print)
Keywords: isolation, culture, protoplasts, plant regeneration, winged bean
Subjects:

ePrint ID: 75722
Date :
Date Event
3 October 1985Published
Date Deposited: 11 Mar 2010
Last Modified: 18 Apr 2017 20:42
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/75722

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