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Protoplast isolation, culture and plant regeneration in the winged bean, Psophocarpus tetragonolobus (L) D.C.

Record type: Article

Protoplasts have been isolated from winged bean (Psophocarpus tetragonolobus) suspension culture cells using a 2-mercaptoethanol supplemented enzyme solution. These protoplasts can be induced to divide and by continued proliferation give rise to callus colonies. Shoots have been regenerated from this callus. These shoots can be rooted and subsequently the plantlets develop to maturity.

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Citation

Wilson, V.M., Haq, N. and Evans, P.K. (1985) Protoplast isolation, culture and plant regeneration in the winged bean, Psophocarpus tetragonolobus (L) D.C. Plant Science, 41, (1), pp. 61-68. (doi:10.1016/0168-9452(85)90066-4).

More information

Published date: 3 October 1985
Keywords: isolation, culture, protoplasts, plant regeneration, winged bean

Identifiers

Local EPrints ID: 75722
URI: http://eprints.soton.ac.uk/id/eprint/75722
ISSN: 0168-9452
PURE UUID: 7f7b90d2-1314-4387-9468-da9423b90804

Catalogue record

Date deposited: 11 Mar 2010
Last modified: 18 Jul 2017 23:42

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Contributors

Author: V.M. Wilson
Author: N. Haq
Author: P.K. Evans

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