Separation of molecular size classes of aquatic humic substances using ultrafiltration and dialysis
Separation of molecular size classes of aquatic humic substances using ultrafiltration and dialysis
A procedure for separating aquatic humic substances into discrete molecular size fractions by the combined use of ultrafiltration and dialysis was tested. The molecular size distributions of separated fractions were examined by application of samples to a gel filtration chromatography column. Variations in the elution patterns observed for the separated fractions of small and intermediate molecular size were largely consistent with predicted effects of separation procedures. In contrast, humic substances of large molecular size were not separated effectively by dialysis using dialysis membranes of large molecular weight cut-off. Small quantities of humic material of large molecular size were observed in most of the ultrafiltrates. This phenomenon may have been an artefact of filtration and/or the result of reconfiguration of material after separation.
humic substances, separation, gel filtration, ultrafiltration, dialysis
765-774
Shaw, Peter J.
935dfebf-9fb6-483c-86da-a21dba8c1989
Jones, Roger I.
a5a845e7-405b-4793-a9c7-e2508d64d884
De Haan, Henk
bde49bc5-b4fe-4dfc-8c83-7f6a6a652254
August 1994
Shaw, Peter J.
935dfebf-9fb6-483c-86da-a21dba8c1989
Jones, Roger I.
a5a845e7-405b-4793-a9c7-e2508d64d884
De Haan, Henk
bde49bc5-b4fe-4dfc-8c83-7f6a6a652254
Shaw, Peter J., Jones, Roger I. and De Haan, Henk
(1994)
Separation of molecular size classes of aquatic humic substances using ultrafiltration and dialysis.
Environmental Technology, 15 (8), .
Abstract
A procedure for separating aquatic humic substances into discrete molecular size fractions by the combined use of ultrafiltration and dialysis was tested. The molecular size distributions of separated fractions were examined by application of samples to a gel filtration chromatography column. Variations in the elution patterns observed for the separated fractions of small and intermediate molecular size were largely consistent with predicted effects of separation procedures. In contrast, humic substances of large molecular size were not separated effectively by dialysis using dialysis membranes of large molecular weight cut-off. Small quantities of humic material of large molecular size were observed in most of the ultrafiltrates. This phenomenon may have been an artefact of filtration and/or the result of reconfiguration of material after separation.
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Published date: August 1994
Keywords:
humic substances, separation, gel filtration, ultrafiltration, dialysis
Identifiers
Local EPrints ID: 75747
URI: http://eprints.soton.ac.uk/id/eprint/75747
ISSN: 0959-3330
PURE UUID: 0ec977ed-0a8a-4234-b906-03fc3dfd2044
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Date deposited: 11 Mar 2010
Last modified: 11 Dec 2021 03:03
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Contributors
Author:
Roger I. Jones
Author:
Henk De Haan
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