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Ovarian stimulation for in vitro fertilization alters the intrauterine cytokine, chemokine, and growth factor milieu encountered by the embryo

Ovarian stimulation for in vitro fertilization alters the intrauterine cytokine, chemokine, and growth factor milieu encountered by the embryo
Ovarian stimulation for in vitro fertilization alters the intrauterine cytokine, chemokine, and growth factor milieu encountered by the embryo
Objective: to elucidate the impact of ovarian stimulation on the intrauterine milieu represented by the cytokine, chemokine, and growth factor profile in endometrial secretions aspirated before embryo transfer.

Design: prospective cohort study.

Setting: fertility center in tertiary referral university hospital.

Patient(s): forty-two patients undergoing ovarian stimulation with GnRH analogues were recruited. They participated in both a natural and an ovarian-stimulated cycle for within patient comparisons.

Interventions(s): endometrial secretion aspiration was performed immediately before embryo transfer.

Main outcome measure(s): the concentrations of 17 mediators known to be involved in human embryo implantation were assessed by multiplex immunoassay.

Result(s): after correction for multiple testing, significantly higher concentrations of interleukin (IL)-1beta, IL-5, IL-10, IL-12, IL-17, tumor necrosis factor (TNF)-alpha, heparin-binding epidermal growth factor (HbEGF), eotaxin, and dickkopf homologue-1 were present in endometrial secretions obtained in stimulated compared with natural cycles.

Conclusion(s): endometrial secretion analysis provides a novel means of investigating the effect of ovarian stimulation on the intrauterine milieu. The in vivo milieu encountered by the embryo after transfer is significantly altered by ovarian stimulation.
ovarian stimulation, endometrium, cytokine, in vitro fertilization
0015-0282
1764-1768
Boomsma, Carolien M.
d5c20848-2432-47ce-b262-ad35407a64b9
Kavelaars, Annemieke
ab4a504e-74de-4c7b-86b4-acac8363cc77
Eijkemans, Marinus J.C.
f95640f3-5107-4b1b-b924-ebac8a334321
Fauser, Bart C.J.M.
0edf5e0f-12db-4ca9-bf9c-00e85b7f4a3e
Heijnen, Cobi J.
7327b3c6-a13d-4569-8a8a-f16dec3d9292
Macklon, Nick S.
7db1f4fc-a9f6-431f-a1f2-297bb8c9fb7e
Boomsma, Carolien M.
d5c20848-2432-47ce-b262-ad35407a64b9
Kavelaars, Annemieke
ab4a504e-74de-4c7b-86b4-acac8363cc77
Eijkemans, Marinus J.C.
f95640f3-5107-4b1b-b924-ebac8a334321
Fauser, Bart C.J.M.
0edf5e0f-12db-4ca9-bf9c-00e85b7f4a3e
Heijnen, Cobi J.
7327b3c6-a13d-4569-8a8a-f16dec3d9292
Macklon, Nick S.
7db1f4fc-a9f6-431f-a1f2-297bb8c9fb7e

Boomsma, Carolien M., Kavelaars, Annemieke, Eijkemans, Marinus J.C., Fauser, Bart C.J.M., Heijnen, Cobi J. and Macklon, Nick S. (2009) Ovarian stimulation for in vitro fertilization alters the intrauterine cytokine, chemokine, and growth factor milieu encountered by the embryo. Fertility and Sterility, 94 (5), 1764-1768. (doi:10.1016/j.fertnstert.2009.10.044).

Record type: Article

Abstract

Objective: to elucidate the impact of ovarian stimulation on the intrauterine milieu represented by the cytokine, chemokine, and growth factor profile in endometrial secretions aspirated before embryo transfer.

Design: prospective cohort study.

Setting: fertility center in tertiary referral university hospital.

Patient(s): forty-two patients undergoing ovarian stimulation with GnRH analogues were recruited. They participated in both a natural and an ovarian-stimulated cycle for within patient comparisons.

Interventions(s): endometrial secretion aspiration was performed immediately before embryo transfer.

Main outcome measure(s): the concentrations of 17 mediators known to be involved in human embryo implantation were assessed by multiplex immunoassay.

Result(s): after correction for multiple testing, significantly higher concentrations of interleukin (IL)-1beta, IL-5, IL-10, IL-12, IL-17, tumor necrosis factor (TNF)-alpha, heparin-binding epidermal growth factor (HbEGF), eotaxin, and dickkopf homologue-1 were present in endometrial secretions obtained in stimulated compared with natural cycles.

Conclusion(s): endometrial secretion analysis provides a novel means of investigating the effect of ovarian stimulation on the intrauterine milieu. The in vivo milieu encountered by the embryo after transfer is significantly altered by ovarian stimulation.

Full text not available from this repository.

More information

Published date: 10 December 2009
Keywords: ovarian stimulation, endometrium, cytokine, in vitro fertilization
Organisations: Dev Origins of Health & Disease, Human Development & Health

Identifiers

Local EPrints ID: 76214
URI: https://eprints.soton.ac.uk/id/eprint/76214
ISSN: 0015-0282
PURE UUID: 6c40ed6b-bafd-438e-83a2-aca9518d4752

Catalogue record

Date deposited: 11 Mar 2010
Last modified: 17 Jul 2019 00:15

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