The University of Southampton
University of Southampton Institutional Repository

Endometrial secretion analysis identifies a cytokine profile predictive of pregnancy in IVF

Boomsma, C.M., Kavelaars, A., Eijkemans, M.J., Lentjes, E.G., Fauser, B.C., Heijnen, C.J. and Macklon, N.S. (2009) Endometrial secretion analysis identifies a cytokine profile predictive of pregnancy in IVF Human Reproduction, 24, (6), pp. 1427-1435. (doi:10.1093/humrep/dep011).

Record type: Article


Background: The study of human endometrial–embryonic interactions is complicated by the disruptive impact of endometrial sample collection on the process of implantation itself. Endometrial secretion analysis is a novel technique, non-disruptive to implantation. The primary aim of this prospective cohort study was to explore whether a cytokine profile predictive of implantation and clinical pregnancy can be identified in endometrial secretions aspirated immediately prior to embryo transfer following IVF.
Methods: Endometrial secretions, aspirated immediately prior to embryo transfer from 210 women undergoing IVF, were analyzed using a multiplex immunoassay for 17 soluble regulators of implantation, namely interleukin (IL)-1?, IL-5, IL-6, IL-10, IL-12, IL-15, IL-17, IL-18, tumor necrosis factor (TNF)-, interferon (IFN)-, macrophage migration inhibitory factor, eotaxin, IFN--inducible 10 kDa protein (IP-10), monocyte chemo-attractant protein-1 (MCP-1), Dickkopf homolog 1, heparin-binding epidermal growth factor and vascular endothelial growth factor (VEGF). In order to detect implantation, daily urine samples were collected after embryo transfer, and human Chorionic Gonadotropin (hCG) concentrations were analyzed by an immunoassay.
Results: Multivariable logistic regression analysis revealed significant associations (negative and positive association, respectively) between MCP-1 (P = 0.005) and IP-10 (P = 0.037) levels and implantation, and between IL-1? (P = 0.047) and TNF- (P = 0.023) levels and clinical pregnancy. The predictive value for pregnancy of IL-1? and TNF- was observed to be equivalent and additive to that of embryo quality.
Conclusions: Endometrial secretion cytokine profiling offers a novel, non-disruptive approach to study the role of the endometrium in human embryo implantation and identifies a profile which appears to be conducive to clinical pregnancy.

Full text not available from this repository.

More information

Published date: June 2009
Keywords: cytokines, pregnancy, IVF, ICSI, outcome, endometrial receptivity, implantation
Organisations: Dev Origins of Health & Disease


Local EPrints ID: 76215
PURE UUID: 902c99c5-3464-43d1-97f6-85e6d6a6e33e

Catalogue record

Date deposited: 15 Mar 2010
Last modified: 18 Jul 2017 23:41

Export record



Author: C.M. Boomsma
Author: A. Kavelaars
Author: M.J. Eijkemans
Author: E.G. Lentjes
Author: B.C. Fauser
Author: C.J. Heijnen
Author: N.S. Macklon

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.