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Homocysteine metabolism in the pre-ovulatory follicle during ovarian stimulation

Boxmeer, Jolanda C., Steegers-Theunissen, Régine P.M., Lindemans, Jan, Wildhagen, Mark F., Martini, Elena, Steegers, Eric A.P. and Macklon, Nick S. (2008) Homocysteine metabolism in the pre-ovulatory follicle during ovarian stimulation Human Reproduction, 23, (11), pp. 2570-2576. (doi:10.1093/humrep/den292).

Record type: Article


BACKGROUND: Ovarian stimulation gives rise to supraphysiological estradiol levels, which may affect oocyte quality. This study aims to investigate whether ovarian stimulation deranges the homocysteine pathway thereby affecting the pre-ovulatory follicle.
METHODS: Blood samples were collected on cycle day 2 and the day of hCG administration in 181 women undergoing ovarian stimulation for IVF. In each subject, the diameter of the two leading follicles was measured and the corresponding follicular fluids were collected. In blood and follicular fluid samples, total homocysteine (tHcy), folate, cobalamin and pyridoxal’5-phosphate (PLP) were determined. According to the blood folate levels, women were classified as either folic acid supplemented (n = 113) or non-supplemented (n = 32).
RESULTS: Ovarian stimulation resulted in a significant decrease in blood tHcy and cobalamin levels (both P ? 0.001). The blood concentrations of tHcy, folate, cobalamin and PLP were significantly correlated with the corresponding follicular fluid concentrations (all P ? 0.001). Follicular fluid tHcy concentrations were inversely correlated with follicular diameter (P ? 0.05). In folic acid supplemented women, follicular fluid folate was inversely correlated with follicular diameter (P ? 0.05).
CONCLUSIONS: Ovarian stimulation deranges blood and follicular fluid biomarkers of the homocysteine pathway. High ovarian follicular fluid tHcy and folate levels may have detrimental effects on follicular development

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Published date: November 2008
Keywords: folic acid, cobalamin, pyridoxine, assisted reproduction, follicular fluid


Local EPrints ID: 76217
PURE UUID: 9113c024-c92d-4905-b2bd-7fb1c4a62c59

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Date deposited: 15 Mar 2010
Last modified: 18 Jul 2017 23:41

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Author: Jolanda C. Boxmeer
Author: Régine P.M. Steegers-Theunissen
Author: Jan Lindemans
Author: Mark F. Wildhagen
Author: Elena Martini
Author: Eric A.P. Steegers
Author: Nick S. Macklon

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