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Sensitivity enhancement of transducers for total internal reflection fluorescence

Sensitivity enhancement of transducers for total internal reflection fluorescence
Sensitivity enhancement of transducers for total internal reflection fluorescence
We have developed, modeled and optimized optical transducers for total internal reflection fluorescence (TIRF). The transducers are part of a compact and rugged immuno-analytical instrument designed for simultaneous detection of up to six analytes in aquatic samples (e.g. atrazine and 2,4-D). Binding inhibition assays, using Cy5.5 labeled antibodies to detect the target analytes, have been carried out. Calibration curves with mid-points of tests below 1 µg/1 and detection limits below 0.1 µg/1 have been achieved. As transducer either ion exchanged integrated optical channel waveguides or planar multimode slab waveguides have been employed. The transducer performance was significantly enhanced by incorporating thin high index films at the waveguide surface and by applying high refractive index solutions in the superstrate. Peak signal enhancement factors of more than ten have been observed and an increase in signal to noise ratio by a factor of more than four have been achieved. Strong polarization dependent effects on the enhancement by high index films have been found both theoretically and experimentally.
345
Society of Photo-Optical Instrumentation Engineers
Klotz, Albrecht
7bd3b909-ff62-449a-bb4e-b9f7cc90b273
Barzen, C.
e57de777-3451-4b75-b44a-937e8a317e5b
Brecht, Andreas
a89076d3-ab3d-499a-abfc-7842a8ec6157
Harris, Richard D.
485577e2-e094-4ff8-a19f-73df39e47ce4
Quigley, G
21cb49e2-2e3e-4306-b6b9-52e93278cc74
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Gauglitz, Guenter
c7a244e5-e7dc-4dce-a9fd-c83945597956
Righini, Giancarlo C.
Najafi, S. Iraj
Klotz, Albrecht
7bd3b909-ff62-449a-bb4e-b9f7cc90b273
Barzen, C.
e57de777-3451-4b75-b44a-937e8a317e5b
Brecht, Andreas
a89076d3-ab3d-499a-abfc-7842a8ec6157
Harris, Richard D.
485577e2-e094-4ff8-a19f-73df39e47ce4
Quigley, G
21cb49e2-2e3e-4306-b6b9-52e93278cc74
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Gauglitz, Guenter
c7a244e5-e7dc-4dce-a9fd-c83945597956
Righini, Giancarlo C.
Najafi, S. Iraj

Klotz, Albrecht, Barzen, C., Brecht, Andreas, Harris, Richard D., Quigley, G, Wilkinson, James S. and Gauglitz, Guenter (1999) Sensitivity enhancement of transducers for total internal reflection fluorescence. Righini, Giancarlo C. and Najafi, S. Iraj (eds.) In Sensors. vol. 3620, Society of Photo-Optical Instrumentation Engineers. p. 345 . (doi:10.1117/12.343754).

Record type: Conference or Workshop Item (Paper)

Abstract

We have developed, modeled and optimized optical transducers for total internal reflection fluorescence (TIRF). The transducers are part of a compact and rugged immuno-analytical instrument designed for simultaneous detection of up to six analytes in aquatic samples (e.g. atrazine and 2,4-D). Binding inhibition assays, using Cy5.5 labeled antibodies to detect the target analytes, have been carried out. Calibration curves with mid-points of tests below 1 µg/1 and detection limits below 0.1 µg/1 have been achieved. As transducer either ion exchanged integrated optical channel waveguides or planar multimode slab waveguides have been employed. The transducer performance was significantly enhanced by incorporating thin high index films at the waveguide surface and by applying high refractive index solutions in the superstrate. Peak signal enhancement factors of more than ten have been observed and an increase in signal to noise ratio by a factor of more than four have been achieved. Strong polarization dependent effects on the enhancement by high index films have been found both theoretically and experimentally.

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More information

Published date: 1999
Venue - Dates: International Symposium on Integrated Optoelectronic Devices, San Jose City, Philippines, 1999-01-23 - 1999-01-29

Identifiers

Local EPrints ID: 76496
URI: http://eprints.soton.ac.uk/id/eprint/76496
PURE UUID: 1c85a140-ac44-4ce4-8ed5-9178f683f063
ORCID for James S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 11 Mar 2010
Last modified: 03 Aug 2021 01:32

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Contributors

Author: Albrecht Klotz
Author: C. Barzen
Author: Andreas Brecht
Author: Richard D. Harris
Author: G Quigley
Author: Guenter Gauglitz
Editor: Giancarlo C. Righini
Editor: S. Iraj Najafi

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