Integrated optical surface plasmon resonance immunoprobe for simazine detection
Integrated optical surface plasmon resonance immunoprobe for simazine detection
This paper presents the detailed design and characterisation of a regenerable integrated optical surface plasmon resonance immunoprobe as a detector for the triazine herbicide simazine. A sensor design theoretically optimised for use in the aqueous environment is presented and its fabrication described. Experimental results on the sensitivity to changes in bulk refractive index of the analyte and on non-specific binding of ovalbumin are presented. Binding inhibition immunoassays were conducted for simazine and the limit of detection determined to be 0.16µg/l using anti-simazine IgG antibodies and 0.11µg/l using anti-simazine Fab fragments. A sample test cycle of 20 minutes was established.
surface plasmon resonance, integrated optics, immunoprobe, environmental monitoring
377-386
Harris, R.D.
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Luff, B.J.
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Wilkinson, J.S.
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Piehler, J.
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Brecht, A.
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Gauglitz, G.
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Abuknesha, R.A.
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30 April 1999
Harris, R.D.
e972b676-3335-44cd-b6e8-0dae17c550c3
Luff, B.J.
04d280ab-ae84-4715-9dad-6a1120050ccd
Wilkinson, J.S.
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Piehler, J.
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Brecht, A.
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Gauglitz, G.
119b102d-52db-4eba-81c0-0bdd5a16f1e7
Abuknesha, R.A.
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Harris, R.D., Luff, B.J., Wilkinson, J.S., Piehler, J., Brecht, A., Gauglitz, G. and Abuknesha, R.A.
(1999)
Integrated optical surface plasmon resonance immunoprobe for simazine detection.
Biosensors & Bioelectronics, 14 (4), .
(doi:10.1016/S0956-5663(99)00014-7).
Abstract
This paper presents the detailed design and characterisation of a regenerable integrated optical surface plasmon resonance immunoprobe as a detector for the triazine herbicide simazine. A sensor design theoretically optimised for use in the aqueous environment is presented and its fabrication described. Experimental results on the sensitivity to changes in bulk refractive index of the analyte and on non-specific binding of ovalbumin are presented. Binding inhibition immunoassays were conducted for simazine and the limit of detection determined to be 0.16µg/l using anti-simazine IgG antibodies and 0.11µg/l using anti-simazine Fab fragments. A sample test cycle of 20 minutes was established.
Text
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- Author's Original
More information
Published date: 30 April 1999
Keywords:
surface plasmon resonance, integrated optics, immunoprobe, environmental monitoring
Identifiers
Local EPrints ID: 77848
URI: http://eprints.soton.ac.uk/id/eprint/77848
ISSN: 0956-5663
PURE UUID: b3891a0a-9164-4a2c-93ba-32c235d30f7e
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Date deposited: 11 Mar 2010
Last modified: 14 Mar 2024 02:32
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Contributors
Author:
R.D. Harris
Author:
B.J. Luff
Author:
J. Piehler
Author:
A. Brecht
Author:
G. Gauglitz
Author:
R.A. Abuknesha
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