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Integrated optical surface plasmon resonance immunoprobe for simazine detection

Integrated optical surface plasmon resonance immunoprobe for simazine detection
Integrated optical surface plasmon resonance immunoprobe for simazine detection
This paper presents the detailed design and characterisation of a regenerable integrated optical surface plasmon resonance immunoprobe as a detector for the triazine herbicide simazine. A sensor design theoretically optimised for use in the aqueous environment is presented and its fabrication described. Experimental results on the sensitivity to changes in bulk refractive index of the analyte and on non-specific binding of ovalbumin are presented. Binding inhibition immunoassays were conducted for simazine and the limit of detection determined to be 0.16µg/l using anti-simazine IgG antibodies and 0.11µg/l using anti-simazine Fab fragments. A sample test cycle of 20 minutes was established.
surface plasmon resonance, integrated optics, immunoprobe, environmental monitoring
0956-5663
377-386
Harris, R.D.
e972b676-3335-44cd-b6e8-0dae17c550c3
Luff, B.J.
04d280ab-ae84-4715-9dad-6a1120050ccd
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Piehler, J.
c7ccd012-3ad1-4b8a-aa0c-6244ab00630f
Brecht, A.
1b34ee2a-ada5-48ec-923a-f385835fbc83
Gauglitz, G.
119b102d-52db-4eba-81c0-0bdd5a16f1e7
Abuknesha, R.A.
f8222e58-66df-41c8-912f-1a11b2c3db6d
Harris, R.D.
e972b676-3335-44cd-b6e8-0dae17c550c3
Luff, B.J.
04d280ab-ae84-4715-9dad-6a1120050ccd
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Piehler, J.
c7ccd012-3ad1-4b8a-aa0c-6244ab00630f
Brecht, A.
1b34ee2a-ada5-48ec-923a-f385835fbc83
Gauglitz, G.
119b102d-52db-4eba-81c0-0bdd5a16f1e7
Abuknesha, R.A.
f8222e58-66df-41c8-912f-1a11b2c3db6d

Harris, R.D., Luff, B.J., Wilkinson, J.S., Piehler, J., Brecht, A., Gauglitz, G. and Abuknesha, R.A. (1999) Integrated optical surface plasmon resonance immunoprobe for simazine detection. Biosensors & Bioelectronics, 14 (4), 377-386. (doi:10.1016/S0956-5663(99)00014-7).

Record type: Article

Abstract

This paper presents the detailed design and characterisation of a regenerable integrated optical surface plasmon resonance immunoprobe as a detector for the triazine herbicide simazine. A sensor design theoretically optimised for use in the aqueous environment is presented and its fabrication described. Experimental results on the sensitivity to changes in bulk refractive index of the analyte and on non-specific binding of ovalbumin are presented. Binding inhibition immunoassays were conducted for simazine and the limit of detection determined to be 0.16µg/l using anti-simazine IgG antibodies and 0.11µg/l using anti-simazine Fab fragments. A sample test cycle of 20 minutes was established.

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More information

Published date: 30 April 1999
Keywords: surface plasmon resonance, integrated optics, immunoprobe, environmental monitoring

Identifiers

Local EPrints ID: 77848
URI: http://eprints.soton.ac.uk/id/eprint/77848
ISSN: 0956-5663
PURE UUID: b3891a0a-9164-4a2c-93ba-32c235d30f7e
ORCID for J.S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 11 Mar 2010
Last modified: 07 Oct 2020 02:12

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