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Integrated optical Mach-Zehnder interferometer as simazine immunoprobe

Integrated optical Mach-Zehnder interferometer as simazine immunoprobe
Integrated optical Mach-Zehnder interferometer as simazine immunoprobe
Immunoassay has become a versatile tool in several fields of analytical chemistry. We describe the characterization and the application of different integrated optical channel waveguide Mach-Zehnder interferometers (MZIs) as label-free immunoprobes. The performance of the classical MZI is compared with that of a modified structure which incorporates a 3x3 coupler. Characterization of the devices demonstrates a dramatic improvement gained by using the 3x3 coupler. Two main advantages are achieved by the modified device. First, the possibility of referencing the output signal allows the elimination of signal fluctuations due to coupling and light-source instabilities. An increase of the signal-to-noise ratio by a factor of up to 10 is achieved. Secondly, the phase shift between the three outputs allows unambiguous detection with optimum sensitivity. For the detection of the herbicide simazine, the functional properties of the transducer surface are optimized by an appropriate chemical modification. Using this improved device, a simazine immunoassay has been carried out with a test midpoint of 0.3 ppb and a detection limit of approximately 0.1 ppb. The excellent performance, established manufacturing techniques and the potential for simplification and parallelization make the device attractive for further development.
0925-4005
277-282
Drapp, B.
4d1de1b5-bd83-4096-9dfa-35cf2959534f
Piehler, J.
c7ccd012-3ad1-4b8a-aa0c-6244ab00630f
Brecht, A.
1b34ee2a-ada5-48ec-923a-f385835fbc83
Gauglitz, G.
119b102d-52db-4eba-81c0-0bdd5a16f1e7
Luff, B.J.
04d280ab-ae84-4715-9dad-6a1120050ccd
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Ingenhoff, J.
222a9715-7807-4f92-8001-81789ee165de
Drapp, B.
4d1de1b5-bd83-4096-9dfa-35cf2959534f
Piehler, J.
c7ccd012-3ad1-4b8a-aa0c-6244ab00630f
Brecht, A.
1b34ee2a-ada5-48ec-923a-f385835fbc83
Gauglitz, G.
119b102d-52db-4eba-81c0-0bdd5a16f1e7
Luff, B.J.
04d280ab-ae84-4715-9dad-6a1120050ccd
Wilkinson, J.S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Ingenhoff, J.
222a9715-7807-4f92-8001-81789ee165de

Drapp, B., Piehler, J., Brecht, A., Gauglitz, G., Luff, B.J., Wilkinson, J.S. and Ingenhoff, J. (1997) Integrated optical Mach-Zehnder interferometer as simazine immunoprobe. Sensors and Actuators B: Chemical, 39 (1-3), 277-282. (PMID:10.1016/S0925-4005(97)80218-3)

Record type: Article

Abstract

Immunoassay has become a versatile tool in several fields of analytical chemistry. We describe the characterization and the application of different integrated optical channel waveguide Mach-Zehnder interferometers (MZIs) as label-free immunoprobes. The performance of the classical MZI is compared with that of a modified structure which incorporates a 3x3 coupler. Characterization of the devices demonstrates a dramatic improvement gained by using the 3x3 coupler. Two main advantages are achieved by the modified device. First, the possibility of referencing the output signal allows the elimination of signal fluctuations due to coupling and light-source instabilities. An increase of the signal-to-noise ratio by a factor of up to 10 is achieved. Secondly, the phase shift between the three outputs allows unambiguous detection with optimum sensitivity. For the detection of the herbicide simazine, the functional properties of the transducer surface are optimized by an appropriate chemical modification. Using this improved device, a simazine immunoassay has been carried out with a test midpoint of 0.3 ppb and a detection limit of approximately 0.1 ppb. The excellent performance, established manufacturing techniques and the potential for simplification and parallelization make the device attractive for further development.

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Published date: March 1997
Additional Information: 3rd European Conference on Optical Chemical Sensors and Biosensors

Identifiers

Local EPrints ID: 78058
URI: https://eprints.soton.ac.uk/id/eprint/78058
ISSN: 0925-4005
PURE UUID: 3106bdf2-3691-4d79-a8bd-1ae255c254eb
ORCID for J.S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

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Date deposited: 11 Mar 2010
Last modified: 20 Jul 2019 01:29

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