Mouvet, C., Harris, R.D., Maciag, C., Luff, B.J., Wilkinson, J.S., Piehler, J., Brecht, A., Gauglitz, G., Abuknesha, R. and Ismail, G.
Determination of simazine in water samples by waveguide surface plasmon resonance
Analytica Chimica Acta, 338, (1-2), . (doi:10.1016/S0003-2670(96)00443-6).
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We assessed a new sensing device based on the monitoring of immunobinding reactions using Waveguide Surface Plasmon Resonance (WSPR) for the determination of simazine in water samples. Standard solutions between 0.1 and 1.0µg/l analysed in triplicate showed a mean within-day variability of 5%. Calibration curves for the same standards conducted on 5 consecutive days showed a 14% mean day-to-day variability. The detection limit calculated as 3 standard deviations below the mean blank value was 0.2µg/l. The upper limit of the working range calculated as a 90% decrease in the blank signal was 2.4µg/l. The cross-reactivity of atrazine and terbuthylazine was 61 and 67%, respectively. The recovery from spiked natural ground- and surface-water samples ranged from 55 to 153% for spikes ranging from 0.1 to 1.0µg/l. For the 11 surface- and 8 ground-water samples tested, the correlation coefficient between WSPR and HPLC/GC values was significant (p < 0.05) when the chromatography values were calculated as the weighted sum of simazine and atrazine, taking into account the predetermined cross-reactivity of the latter in the WSPR determination. The present system is therefore better suited for screening groups of pesticides than for the determination of a single molecule. An attempt at analysing a soil water sample proved unsuccessful due to interference probably resulting from strong non-selective polyanion-polycation binding to the transducer surface which includes a basic aminodextran. The total duration of one determination, 22 minutes, enables almost immediate measurements without any sample pretreatment other than 0.45µm filtration. No significant alteration of the sensor was observed after 200 determinations.
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