Electrochemiluminescence detection of glucose-oxidase as a model for flow-injection immunoassays
Electrochemiluminescence detection of glucose-oxidase as a model for flow-injection immunoassays
Glucose oxidase was covalently attached to an aminosilanized indium tin oxide coated glass wafer with the hydroxysuccinimide ester of pyridyldithiopropionic acid. The wafer was used as the working electrode in the reaction chamber of a flow injection analyser. When a solution of glucose and luminol was passed over the electrode, glucose was oxidized enzymatically and luminol was oxidized electro-chemically. The products of these reactions generated light in proportion to the amount of glucose in the sample. When glucose was assayed in the range 0-10 mM the detection limit was 0.419 mM with a correlation coefficient of 0.9962.
805-810
Wilson, Robert
86279e42-af90-4ddd-b5ab-5444a0d69ca9
Kremeskotter, Jens
32095a83-587b-4d2d-a744-12ff59deea16
Schiffrin, David J.
9e52b03b-622d-4a73-99cd-05bd462b39fa
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
1996
Wilson, Robert
86279e42-af90-4ddd-b5ab-5444a0d69ca9
Kremeskotter, Jens
32095a83-587b-4d2d-a744-12ff59deea16
Schiffrin, David J.
9e52b03b-622d-4a73-99cd-05bd462b39fa
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Wilson, Robert, Kremeskotter, Jens, Schiffrin, David J. and Wilkinson, James S.
(1996)
Electrochemiluminescence detection of glucose-oxidase as a model for flow-injection immunoassays.
Biosensors & Bioelectronics, 11 (8), .
(doi:10.1016/0956-5663(96)85932-X).
Abstract
Glucose oxidase was covalently attached to an aminosilanized indium tin oxide coated glass wafer with the hydroxysuccinimide ester of pyridyldithiopropionic acid. The wafer was used as the working electrode in the reaction chamber of a flow injection analyser. When a solution of glucose and luminol was passed over the electrode, glucose was oxidized enzymatically and luminol was oxidized electro-chemically. The products of these reactions generated light in proportion to the amount of glucose in the sample. When glucose was assayed in the range 0-10 mM the detection limit was 0.419 mM with a correlation coefficient of 0.9962.
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Published date: 1996
Identifiers
Local EPrints ID: 78164
URI: http://eprints.soton.ac.uk/id/eprint/78164
ISSN: 0956-5663
PURE UUID: cc5864ef-5f38-4d36-ae94-cee7b919659b
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Date deposited: 11 Mar 2010
Last modified: 14 Mar 2024 02:32
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Author:
Robert Wilson
Author:
Jens Kremeskotter
Author:
David J. Schiffrin
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