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Electrochemiluminescence detection of glucose-oxidase as a model for flow-injection immunoassays

Record type: Article

Glucose oxidase was covalently attached to an aminosilanized indium tin oxide coated glass wafer with the hydroxysuccinimide ester of pyridyldithiopropionic acid. The wafer was used as the working electrode in the reaction chamber of a flow injection analyser. When a solution of glucose and luminol was passed over the electrode, glucose was oxidized enzymatically and luminol was oxidized electro-chemically. The products of these reactions generated light in proportion to the amount of glucose in the sample. When glucose was assayed in the range 0-10 mM the detection limit was 0.419 mM with a correlation coefficient of 0.9962.

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Citation

Wilson, Robert, Kremeskotter, Jens, Schiffrin, David J. and Wilkinson, James S. (1996) Electrochemiluminescence detection of glucose-oxidase as a model for flow-injection immunoassays Biosensors & Bioelectronics, 11, (8), pp. 805-810. (doi:10.1016/0956-5663(96)85932-X).

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Published date: 1996

Identifiers

Local EPrints ID: 78164
URI: http://eprints.soton.ac.uk/id/eprint/78164
ISSN: 0956-5663
PURE UUID: cc5864ef-5f38-4d36-ae94-cee7b919659b
ORCID for James S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

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Date deposited: 11 Mar 2010
Last modified: 18 Jul 2017 23:26

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Contributors

Author: Robert Wilson
Author: Jens Kremeskotter
Author: David J. Schiffrin

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