The identification of phytoplankton pigments from absorption spectra

Aguirre-Gomez, R., Weeks, A.R. and Boxall, S.R. (2001) The identification of phytoplankton pigments from absorption spectra International Journal of Remote Sensing, 22, (2-3), pp. 315-338. (doi:10.1080/014311601449952).


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The absorption spectra of photosynthetic algae are characterized by a continuous envelope, which is a result of the overlapping spectra of the indivual pigments. This feature makes it difficult to estimate the contribution of each pigment to the total absorption spectra. Derivative analysis is an objective tool for isolating absorption peaks in phytoplankton. Theoretically, electrons and ions of interacting molecules can be regarded as simple harmonic oscillators in an electromagnetic field, which result in a Lorentzian shape. However, when measured by an optical spectrophotometer the signal is transformed into a Gaussian curve. Thus, a combination of both types of curve provides a realistic approach to the decomposition of absorption spectra. In this study derivative analysis is performed on absorption spectra in order to prove that the method can be successfully used to identify the individual absorption spectra of component pigments. The spectra used are modelled phytoplankton, spectrophotometric measurements of algal cultures and samples from natural waters. A combination of Gaussian-Lorentzian shaped curves, centred on the identified peaks, were compared with the original spectra and showed good agreement.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1080/014311601449952
ISSNs: 0143-1161 (print)
Related URLs:
ePrint ID: 7883
Date :
Date Event
Date Deposited: 19 Jul 2004
Last Modified: 17 Apr 2017 00:08
Further Information:Google Scholar

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