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The identification of phytoplankton pigments from absorption spectra

Aguirre-Gomez, R., Weeks, A.R. and Boxall, S.R. (2001) The identification of phytoplankton pigments from absorption spectra International Journal of Remote Sensing, 22, (2-3), pp. 315-338.

Record type: Article

Abstract

The absorption spectra of photosynthetic algae are characterized by a continuous envelope, which is a result of the overlapping spectra of the indivual pigments. This feature makes it difficult to estimate the contribution of each pigment to the total absorption spectra. Derivative analysis is an objective tool for isolating absorption peaks in phytoplankton. Theoretically, electrons and ions of interacting molecules can be regarded as simple harmonic oscillators in an electromagnetic field, which result in a Lorentzian shape. However, when measured by an optical spectrophotometer the signal is transformed into a Gaussian curve. Thus, a combination of both types of curve provides a realistic approach to the decomposition of absorption spectra. In this study derivative analysis is performed on absorption spectra in order to prove that the method can be successfully used to identify the individual absorption spectra of component pigments. The spectra used are modelled phytoplankton, spectrophotometric measurements of algal cultures and samples from natural waters. A combination of Gaussian-Lorentzian shaped curves, centred on the identified peaks, were compared with the original spectra and showed good agreement.

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More information

Published date: 2001
Keywords: PHYTOPLANKTON, PIGMENTS, ABSORPTION SPECTRA

Identifiers

Local EPrints ID: 7883
URI: http://eprints.soton.ac.uk/id/eprint/7883
ISSN: 0143-1161
PURE UUID: c26b9c77-b65c-4b04-8577-ce01a04d40bd

Catalogue record

Date deposited: 19 Jul 2004
Last modified: 19 Oct 2017 20:37

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Contributors

Author: R. Aguirre-Gomez
Author: A.R. Weeks
Author: S.R. Boxall

University divisions

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