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Development of an infrared diagnostic instrument for the measurement of CO2 isotope ratios in breath

Development of an infrared diagnostic instrument for the measurement of CO2 isotope ratios in breath
Development of an infrared diagnostic instrument for the measurement of CO2 isotope ratios in breath
Stable CO2 isotope breath tests are established as a valuable tool in diagnostic and investigative medicine. The instrument conventionally used is an Isotope Ratio Mass Spectrometer, however, the expense and complexity of such an instrument severely restricts the widespread and routine use of isotope tests. To realize their full potential an alternative instrument which is reliable, uncomplicated, insensitive to environmental and component fluctuations and affordable is required. We present a system that satisfies these criteria using broadband non-dispersive infrared spectroscopy. Two isotopically distinct channel pathlengths are recorded for a basal and 13-CO2 enriched breath sample, the change in channel pathlength ratios required to restore transmitted intensity equilibrium between channels being related to the change in the 13-CO2/12-CO2 concentration ratio. The novelty of the system lies in this negative feedback loop balancing the signal by means of adjusting one of the channels pathlength. Results are presented proving the validity of using infrared spectroscopy for making such measurements without interference from breath trace compounds. Theoretical computer simulations using detailed modeling of CO2 spectroscopy have been used to evaluate and quantify the risk posed to reliable measurement of 13-CO2/12-CO2 due to various spectral effects. From this we have established operating tolerances necessary to avoid or limit the generation of a spurious result.
36-48
The International Society for Optical Engineering
Mansfield, C.D.
2354cf62-85aa-497f-8a77-b0705e608bf3
Rutt, H.N.
e09fa327-0c01-467a-9898-4e7f0cd715fc
Cohn, Gerald E.
Owicki, John C.
Mansfield, C.D.
2354cf62-85aa-497f-8a77-b0705e608bf3
Rutt, H.N.
e09fa327-0c01-467a-9898-4e7f0cd715fc
Cohn, Gerald E.
Owicki, John C.

Mansfield, C.D. and Rutt, H.N. (1999) Development of an infrared diagnostic instrument for the measurement of CO2 isotope ratios in breath. In, Cohn, Gerald E. and Owicki, John C. (eds.) Systems and Technologies for Clinical Diagnostics and Drug Discovery II. (Proceedings of SPIE, , (doi:10.1117/12.346757), 3603) Photonics West '99: Systems and Technologies for Clinical Diagnostics and Drug Discovery II (01/01/99) Bellingham, US. The International Society for Optical Engineering, pp. 36-48. (doi:10.1117/12.346757).

Record type: Book Section

Abstract

Stable CO2 isotope breath tests are established as a valuable tool in diagnostic and investigative medicine. The instrument conventionally used is an Isotope Ratio Mass Spectrometer, however, the expense and complexity of such an instrument severely restricts the widespread and routine use of isotope tests. To realize their full potential an alternative instrument which is reliable, uncomplicated, insensitive to environmental and component fluctuations and affordable is required. We present a system that satisfies these criteria using broadband non-dispersive infrared spectroscopy. Two isotopically distinct channel pathlengths are recorded for a basal and 13-CO2 enriched breath sample, the change in channel pathlength ratios required to restore transmitted intensity equilibrium between channels being related to the change in the 13-CO2/12-CO2 concentration ratio. The novelty of the system lies in this negative feedback loop balancing the signal by means of adjusting one of the channels pathlength. Results are presented proving the validity of using infrared spectroscopy for making such measurements without interference from breath trace compounds. Theoretical computer simulations using detailed modeling of CO2 spectroscopy have been used to evaluate and quantify the risk posed to reliable measurement of 13-CO2/12-CO2 due to various spectral effects. From this we have established operating tolerances necessary to avoid or limit the generation of a spurious result.

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More information

Published date: 1999
Venue - Dates: Photonics West '99: Systems and Technologies for Clinical Diagnostics and Drug Discovery II, United States, 1999-01-01

Identifiers

Local EPrints ID: 78973
URI: http://eprints.soton.ac.uk/id/eprint/78973
PURE UUID: 23d6c1ef-7584-47cb-adf8-8fe0b0d8d248

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Date deposited: 11 Mar 2010
Last modified: 17 Jul 2019 00:12

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