The University of Southampton
University of Southampton Institutional Repository

T cell receptor V beta expression in human intestine: regional variation in postnatal intestine and biased usage in fetal gut

T cell receptor V beta expression in human intestine: regional variation in postnatal intestine and biased usage in fetal gut
T cell receptor V beta expression in human intestine: regional variation in postnatal intestine and biased usage in fetal gut
A panel of T cell receptor V beta specific monoclonal antibodies was used to analyse V beta gene usage at different sites in human postnatal and fetal intestine. In normal small intestine, at a single site, different patients showed expansion of T cells expressing individual V beta s. Lamina propria and epithelial T cells from the same patient showed overlapping but not identical V beta dominance. V beta dominance was also shown in the T cells of the colonic lamina propria. Analysis of two separate regions of intestine from the same patient (5-100 cm apart) showed that T cells expressing a dominant V beta region were often present at both sites. In most patients, however, major biases in T cell V beta usage (two to 12-fold variation) were also apparent between the two sites. Analysis of V beta expression in human fetal intestine also showed considerable skewing, although the most common dominant V beta in postnatal intestine (V beta 22) was never predominant in fetal intestine. Patchy local variation in the expression of individual V beta s therefore occurs against a background of V beta dominance over large regions of the human gut. Furthermore the results from fetal gut show that factors other than luminal antigen control V beta expression in the gut.
1468-3288
190-195
Thomas, R.
6814ea6d-818e-4088-882f-2b87f0147d2a
Schurmann, G.
ba089296-7612-4e9a-9333-685d465da610
Lionetti, P.
5f7b9885-b7bc-4834-abee-70238aa842c8
Pender, S.L.F.
62528b03-ec42-41bb-80fe-48454c2c5242
MacDonald, T.T.
171334aa-638a-42b0-99f6-e860e2f0ca45
Thomas, R.
6814ea6d-818e-4088-882f-2b87f0147d2a
Schurmann, G.
ba089296-7612-4e9a-9333-685d465da610
Lionetti, P.
5f7b9885-b7bc-4834-abee-70238aa842c8
Pender, S.L.F.
62528b03-ec42-41bb-80fe-48454c2c5242
MacDonald, T.T.
171334aa-638a-42b0-99f6-e860e2f0ca45

Thomas, R., Schurmann, G., Lionetti, P., Pender, S.L.F. and MacDonald, T.T. (1996) T cell receptor V beta expression in human intestine: regional variation in postnatal intestine and biased usage in fetal gut. Gut, 38 (2), 190-195. (doi:10.1136/gut.38.2.190).

Record type: Article

Abstract

A panel of T cell receptor V beta specific monoclonal antibodies was used to analyse V beta gene usage at different sites in human postnatal and fetal intestine. In normal small intestine, at a single site, different patients showed expansion of T cells expressing individual V beta s. Lamina propria and epithelial T cells from the same patient showed overlapping but not identical V beta dominance. V beta dominance was also shown in the T cells of the colonic lamina propria. Analysis of two separate regions of intestine from the same patient (5-100 cm apart) showed that T cells expressing a dominant V beta region were often present at both sites. In most patients, however, major biases in T cell V beta usage (two to 12-fold variation) were also apparent between the two sites. Analysis of V beta expression in human fetal intestine also showed considerable skewing, although the most common dominant V beta in postnatal intestine (V beta 22) was never predominant in fetal intestine. Patchy local variation in the expression of individual V beta s therefore occurs against a background of V beta dominance over large regions of the human gut. Furthermore the results from fetal gut show that factors other than luminal antigen control V beta expression in the gut.

This record has no associated files available for download.

More information

Published date: February 1996

Identifiers

Local EPrints ID: 79332
URI: http://eprints.soton.ac.uk/id/eprint/79332
ISSN: 1468-3288
PURE UUID: e699208e-a8b7-4cdb-8e91-1358431bd305
ORCID for S.L.F. Pender: ORCID iD orcid.org/0000-0001-6332-0333

Catalogue record

Date deposited: 11 Mar 2010
Last modified: 14 Mar 2024 02:45

Export record

Altmetrics

Contributors

Author: R. Thomas
Author: G. Schurmann
Author: P. Lionetti
Author: S.L.F. Pender ORCID iD
Author: T.T. MacDonald

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×