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Effects of supernatants from Kupffer cells stimulated with galactosamine and endotoxin on the function of isolated rat hepatocytes

Record type: Article

Activated Kupffer cells may release substances that are involved in liver injury induced by galactosamine and endotoxin. In the present study Kupffer cells were isolated from rat livers, cultured for 24 hours and incubated with galactosamine, endotoxin (LPS) or tumor necrosis factor-alpha for 4 or 24 hours. The Kupffer cell-conditioned media were than added in separate experiments to freshly prepared isolated rat hepatocytes to determine their cytotoxic effect. No significant effects on the rate of protein synthesis, as assessed by the incorporation of 14C-leucine on lactate dehydrogenase enzyme release from hepatocytes during 1 h incubation was found as compared with conditioned media from control Kupffer cells. In further experiments, Kupffer cells incubated for 4 hours with LPS and galactosamine were shown to produce thromboxane B2 and also the potentially cytoprotective prostaglandins PGE2 and small amounts of prostacyclin measured as 6-keto-PGF1 alpha. It is concluded that under the conditions of the present experiments, factors secreted by cultured Kupffer cells have no cytotoxic effects on isolated rat hepatocytes during short-term incubation.

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Citation

Kmiec, Z., Hughes, R., Moore, K., Sheron, N., Gove, C., Nouri-Akia, K.T. and Williams, R. (1993) Effects of supernatants from Kupffer cells stimulated with galactosamine and endotoxin on the function of isolated rat hepatocytes Hepatogastroenterology, 40, (3), pp. 259-261.

More information

Published date: 1993

Identifiers

Local EPrints ID: 80064
URI: http://eprints.soton.ac.uk/id/eprint/80064
ISSN: 0172-6390
PURE UUID: 752e84fb-320f-4a80-a2d0-16b8bcdf58ab
ORCID for N. Sheron: ORCID iD orcid.org/0000-0001-5232-8292

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Date deposited: 24 Mar 2010
Last modified: 18 Jul 2017 23:15

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Contributors

Author: Z. Kmiec
Author: R. Hughes
Author: K. Moore
Author: N. Sheron ORCID iD
Author: C. Gove
Author: K.T. Nouri-Akia
Author: R. Williams

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