Retinoic acid-inducible gene-I mediates late phase induction of TNF-? by lipopolysaccharide
Retinoic acid-inducible gene-I mediates late phase induction of TNF-? by lipopolysaccharide
LPS is the known component of bacterial pathogens that stimulates a number of proinflammatory factors. However, the mechanism of the induction of these factors by LPS has not been fully elucidated. We show here that LPS induces retinoic acid-inducible gene-I (RIG-I) in vitro and in vivo as a result from autocrine secretion of IFN-? in macrophages. TIR-domain-containing adapter-inducing IFN-?-deficient mouse embryo fibroblast (trif–/–) fail to show expression of RIG-I following LPS stimulation. Interference of RIG-I expression short interfering RNA represses the expression of LPS-induced TNF-{alpha}, whereas over-expression of RIG-I leads to the activation of TNF-{alpha} promoter and the induction of TNF-{alpha} expression. LPS- and IFN-?-induced TNF-{alpha} are suppressed in RIG-I-deficient mouse embryo fibroblasts (rig–/–). Thus, RIG-I plays a key role in the expression of TNF-{alpha} in macrophages in response to LPS stimulation, mainly for the late phase LPS-induced expression of TNF-{alpha}.
8011-8019
Wang, Jing
28dffdc1-cb6c-40da-b595-88dc7bc1cf1d
Wu, Su
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Jin, Xin
4e0c839f-0d6c-485e-b0cb-a2d031003e9a
Li, Mingtao
72911ffe-b103-4e10-afad-18a4127dd78d
Chen, Shiyong
b8e4ac62-35e7-4e84-b6c6-019e0ab686f6
Teeling, Jessica L.
fcde1c8e-e5f8-4747-9f3a-6bdb5cd87d0a
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Gu, Jun
fd0220da-8019-4327-92ad-fcadeb9896b4
15 June 2008
Wang, Jing
28dffdc1-cb6c-40da-b595-88dc7bc1cf1d
Wu, Su
9e25df7a-ab65-4923-ac0a-c7590f71f29f
Jin, Xin
4e0c839f-0d6c-485e-b0cb-a2d031003e9a
Li, Mingtao
72911ffe-b103-4e10-afad-18a4127dd78d
Chen, Shiyong
b8e4ac62-35e7-4e84-b6c6-019e0ab686f6
Teeling, Jessica L.
fcde1c8e-e5f8-4747-9f3a-6bdb5cd87d0a
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Gu, Jun
fd0220da-8019-4327-92ad-fcadeb9896b4
Wang, Jing, Wu, Su, Jin, Xin, Li, Mingtao, Chen, Shiyong, Teeling, Jessica L., Perry, V. Hugh and Gu, Jun
(2008)
Retinoic acid-inducible gene-I mediates late phase induction of TNF-? by lipopolysaccharide.
Journal of Immunology, 180 (12), .
(PMID:18523264)
Abstract
LPS is the known component of bacterial pathogens that stimulates a number of proinflammatory factors. However, the mechanism of the induction of these factors by LPS has not been fully elucidated. We show here that LPS induces retinoic acid-inducible gene-I (RIG-I) in vitro and in vivo as a result from autocrine secretion of IFN-? in macrophages. TIR-domain-containing adapter-inducing IFN-?-deficient mouse embryo fibroblast (trif–/–) fail to show expression of RIG-I following LPS stimulation. Interference of RIG-I expression short interfering RNA represses the expression of LPS-induced TNF-{alpha}, whereas over-expression of RIG-I leads to the activation of TNF-{alpha} promoter and the induction of TNF-{alpha} expression. LPS- and IFN-?-induced TNF-{alpha} are suppressed in RIG-I-deficient mouse embryo fibroblasts (rig–/–). Thus, RIG-I plays a key role in the expression of TNF-{alpha} in macrophages in response to LPS stimulation, mainly for the late phase LPS-induced expression of TNF-{alpha}.
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Submitted date: 13 September 2007
Published date: 15 June 2008
Organisations:
Biological Sciences
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Local EPrints ID: 80072
URI: http://eprints.soton.ac.uk/id/eprint/80072
ISSN: 0022-1767
PURE UUID: f6fd4d9a-ec0a-4607-abd2-bf8991fc3492
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Date deposited: 24 Mar 2010
Last modified: 14 Mar 2024 02:49
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Author:
Jing Wang
Author:
Su Wu
Author:
Xin Jin
Author:
Mingtao Li
Author:
Shiyong Chen
Author:
Jun Gu
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