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Unaltered SNARE complex formation in an in vivo model of prion disease

Unaltered SNARE complex formation in an in vivo model of prion disease
Unaltered SNARE complex formation in an in vivo model of prion disease
The ME7 model of prion disease is a chronic slowly evolving model of neurodegeneration in which cell death is preceded by synaptic dysfunction. Previous studies in cell culture show that accumulation of misfolded prion inhibits the formation of the SNARE complexes involving synaptobrevin, syntaxin and SNAP-25 that play an essential role in neurotransmitter release. Such observations suggest that similar phenomenon may contribute to synaptic dysfunction observed in vivo. We have thus used detergent extraction of hippocampal tissue to investigate the status of SNARE complexes in the ME7 model. In the presence of increasing PrPSc deposition we failed to see a change in the amount of SNARE complexes directly extracted into SDS and resolved by SDS-PAGE. Conversely pre-extraction in Triton X-100, a treatment that promotes SNARE complexes ex vivo, demonstrated a modest reduction in hippocampal SNARE complexes when homogenates were made from tissue at late stage disease. This suggests that accumulated PrPSc, or perhaps fibrillar complexes formed of prion only inhibit SNARE complexes that are formed ex vivo following biochemical extraction. Thus the accumulation of PrPSc although deleterious to synaptic function in vivo, does not exert its synaptic effects by disrupting the formation of SNARE complexes that are core to transmitter releas.
prion, hippocampus, neurodegeneration, synaptopathy, snare complex, syntaxin, neurotransmitter release
0926-6410
1-7
Asuni, Ayodeji A.
b1412b1b-9794-4705-aada-aed5d3da038f
Cunningham, Colm
3bc1d897-f0f5-4112-abf4-1a10c2e92a6b
Vigneswaran, Piranavhan
4cb42a77-5ccf-4bc0-ad07-3e4d7c2b918b
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
O'Connor, Vincent
8021b06c-01a0-4925-9dde-a61c8fe278ca
Asuni, Ayodeji A.
b1412b1b-9794-4705-aada-aed5d3da038f
Cunningham, Colm
3bc1d897-f0f5-4112-abf4-1a10c2e92a6b
Vigneswaran, Piranavhan
4cb42a77-5ccf-4bc0-ad07-3e4d7c2b918b
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
O'Connor, Vincent
8021b06c-01a0-4925-9dde-a61c8fe278ca

Asuni, Ayodeji A., Cunningham, Colm, Vigneswaran, Piranavhan, Perry, V. Hugh and O'Connor, Vincent (2008) Unaltered SNARE complex formation in an in vivo model of prion disease. Brain Research, 1233, 1-7. (doi:10.1016/j.brainres.2008.07.083).

Record type: Article

Abstract

The ME7 model of prion disease is a chronic slowly evolving model of neurodegeneration in which cell death is preceded by synaptic dysfunction. Previous studies in cell culture show that accumulation of misfolded prion inhibits the formation of the SNARE complexes involving synaptobrevin, syntaxin and SNAP-25 that play an essential role in neurotransmitter release. Such observations suggest that similar phenomenon may contribute to synaptic dysfunction observed in vivo. We have thus used detergent extraction of hippocampal tissue to investigate the status of SNARE complexes in the ME7 model. In the presence of increasing PrPSc deposition we failed to see a change in the amount of SNARE complexes directly extracted into SDS and resolved by SDS-PAGE. Conversely pre-extraction in Triton X-100, a treatment that promotes SNARE complexes ex vivo, demonstrated a modest reduction in hippocampal SNARE complexes when homogenates were made from tissue at late stage disease. This suggests that accumulated PrPSc, or perhaps fibrillar complexes formed of prion only inhibit SNARE complexes that are formed ex vivo following biochemical extraction. Thus the accumulation of PrPSc although deleterious to synaptic function in vivo, does not exert its synaptic effects by disrupting the formation of SNARE complexes that are core to transmitter releas.

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More information

Published date: 3 October 2008
Additional Information: Research report
Keywords: prion, hippocampus, neurodegeneration, synaptopathy, snare complex, syntaxin, neurotransmitter release

Identifiers

Local EPrints ID: 80115
URI: https://eprints.soton.ac.uk/id/eprint/80115
ISSN: 0926-6410
PURE UUID: 9b5e9382-0379-4f30-95a0-d50f68465ecc

Catalogue record

Date deposited: 24 Mar 2010
Last modified: 17 Jul 2019 00:11

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