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Three-dimensional spatial coordinates of individual plankton determined using underwater hologrammetry

Three-dimensional spatial coordinates of individual plankton determined using underwater hologrammetry
Three-dimensional spatial coordinates of individual plankton determined using underwater hologrammetry
We report the use of a nondestructive metrological technique, hologrammetry, that affords greater scope and higher accuracy for the enumeration, sizing, and spatial distribution of particles. Results are presented on imaging of three living planktonic protists suspended in water using in-line pulsed-laser holography. We demonstrate that a volume of 2,400 ml of water, at 750 mm depth, can be analysed with a resolution everywhere better than 20 µm. High quality images and accurate spatial coordinates of living plankton in subvolumes have been obtained.
PARTICLE COUNTERS, HOLOGRAPHY, HOLOGRAMMETRY, OPTICAL PLANKTON RECORDER, PARAMECIUM BURSARIA
0024-3590
1167-1174
Hobson, P.R.
353e4580-b633-46e4-833c-b45387722dd1
Lampitt, R.S.
dfc3785c-fc7d-41fa-89ee-d0c6e27503ad
Rogerson, A.
d8ee58a2-04fd-4a12-ad9f-22b376201cec
Watson, J.
6e817e94-fd80-4a9d-89e9-28d28875b69d
Fang, X.
259a43e1-a422-4a7f-9244-9af2d78cbcba
Krantz, E.P.
01ff38fb-886d-41ce-9d79-c8eec1a9e284
Hobson, P.R.
353e4580-b633-46e4-833c-b45387722dd1
Lampitt, R.S.
dfc3785c-fc7d-41fa-89ee-d0c6e27503ad
Rogerson, A.
d8ee58a2-04fd-4a12-ad9f-22b376201cec
Watson, J.
6e817e94-fd80-4a9d-89e9-28d28875b69d
Fang, X.
259a43e1-a422-4a7f-9244-9af2d78cbcba
Krantz, E.P.
01ff38fb-886d-41ce-9d79-c8eec1a9e284

Hobson, P.R., Lampitt, R.S., Rogerson, A., Watson, J., Fang, X. and Krantz, E.P. (2000) Three-dimensional spatial coordinates of individual plankton determined using underwater hologrammetry. Limnology and Oceanography, 45 (5), 1167-1174.

Record type: Article

Abstract

We report the use of a nondestructive metrological technique, hologrammetry, that affords greater scope and higher accuracy for the enumeration, sizing, and spatial distribution of particles. Results are presented on imaging of three living planktonic protists suspended in water using in-line pulsed-laser holography. We demonstrate that a volume of 2,400 ml of water, at 750 mm depth, can be analysed with a resolution everywhere better than 20 µm. High quality images and accurate spatial coordinates of living plankton in subvolumes have been obtained.

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More information

Published date: 2000
Keywords: PARTICLE COUNTERS, HOLOGRAPHY, HOLOGRAMMETRY, OPTICAL PLANKTON RECORDER, PARAMECIUM BURSARIA

Identifiers

Local EPrints ID: 8956
URI: http://eprints.soton.ac.uk/id/eprint/8956
ISSN: 0024-3590
PURE UUID: 58353c29-1453-4f3e-a368-dc760ecef8df

Catalogue record

Date deposited: 10 Sep 2004
Last modified: 07 Jan 2022 21:58

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Contributors

Author: P.R. Hobson
Author: R.S. Lampitt
Author: A. Rogerson
Author: J. Watson
Author: X. Fang
Author: E.P. Krantz

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