A microfluidic based system for analysis of single cells based on Ca2+ flux (In special issue: Miniaturization)
Zhang, Xunli, Yin, Huabing, Cooper, Jon M. and Haswell, Stephen J. (2006) A microfluidic based system for analysis of single cells based on Ca2+ flux (In special issue: Miniaturization). Electrophoresis, 27, (24), 5093-5100. (doi:10.1002/elps.200600390).
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Description/Abstract
A microfluidic format-based system has been developed for in situ monitoring of the calcium flux response to agonists using Chinese hamster ovary (CHO) cells. The assay is based on measuring the fluorescent intensity of the calcium-sensitive indicator, Fluo-4 AM, and was performed in a modified glass chip channel, whose surface was functionalised using a silanisation method with 3-aminopropyltriethoxysilane (APTS) (enabling the cells to be immobilised on the channel surface). CHO cells calcium flux response was measured for different agonists over a range of concentrations. Cells and reagents were introduced into the chip in a continuous flow as a series of plugs in a given sequence.
| Item Type: | Article |
|---|---|
| ISSNs: | 0173-0835 (print) |
| Related URLs: | |
| Subjects: | T Technology > TP Chemical technology Q Science > QD Chemistry |
| Divisions: | University Structure - Pre August 2011 > School of Engineering Sciences > Bioengineering Sciences |
| Item ID: | 64830 |
| Date Deposited: | 16 Jan 2009 |
| Last Modified: | 02 Mar 2012 13:34 |
| Contributors: | Zhang, Xunli (Author) Yin, Huabing (Author) Cooper, Jon M. (Author) Haswell, Stephen J. (Author) |
| Date: | November 2006 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/64830 |
Available Versions of this Item
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A microfluidic based system for analysis of single cells based on Ca2+ flux. (deposited 24 Oct 2007)
- A microfluidic based system for analysis of single cells based on Ca2+ flux (In special issue: Miniaturization). (deposited 16 Jan 2009) [Currently Displayed]
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