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Conductivity in Jurkat cell suspension after ultrashort electric pulsing

Record type: Conference or Workshop Item (Other)

Ultrashort electric pulses applied to similar cell lines such as Jurkat and HL-60 cells can produce markedly different results , which have been documented extensively over the last few years. We now report changes in electrical conductivity of Jurkat cells subjected to traditional electroporation pulses (50 ms pulse length) and ultrashort pulses (10 ns pulse length) using time domain dielectric spectroscopy (TDS). A single 10 ns, 150 kV/cm pulse did not noticeably alter suspension conductivity while a 50 ms, 2.12 kV/cm pulse with the same energy caused an appreciable conductivity rise. These results support the hypothesis that electroporation pulses primarily interact with the cell membrane and cause conductivity rises due to ion transport from the cell to the external media, while pulses with nanosecond duration primarily interact with the membranes of intracellular organelles. However, multiple ultrashort pulses have a cumulative effect on the plasma membrane, with five pulses causing a gradual rise in conductivity up to ten minutes post-pulsing.

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Citation

Chen, G, Chen, N, Garner, A L, Kolb, J, Swanson, R J, Beebe, S, Joshi, R P and Schoenbach, K H (2004) Conductivity in Jurkat cell suspension after ultrashort electric pulsing At 3rd International workshop on biological effect of EMFs, Greece. 04 - 08 Oct 2004. , pp. 56-65.

More information

Published date: 2004
Additional Information: Event Dates: Oct 4--8, 2004
Venue - Dates: 3rd International workshop on biological effect of EMFs, Greece, 2004-10-04 - 2004-10-08
Keywords: Jurkat cell, electrical conductivity, ultrashort electric pulse
Organisations: Electronics & Computer Science, EEE

Identifiers

Local EPrints ID: 260168
URI: http://eprints.soton.ac.uk/id/eprint/260168
ISBN: 960-233-151-8
PURE UUID: 4d1317e6-b26b-4eb6-a5bf-522149d5933e

Catalogue record

Date deposited: 01 Dec 2004
Last modified: 18 Jul 2017 09:15

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Contributors

Author: G Chen
Author: N Chen
Author: A L Garner
Author: J Kolb
Author: R J Swanson
Author: S Beebe
Author: R P Joshi
Author: K H Schoenbach

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