Chen, G, Chen, N, Garner, A L, Kolb, J, Swanson, R J, Beebe, S, Joshi, R P and Schoenbach, K H
Conductivity in Jurkat cell suspension after ultrashort electric pulsing
At 3rd International workshop on biological effect of EMFs, Greece.
04 - 08 Oct 2004.
Ultrashort electric pulses applied to similar cell lines such as Jurkat and HL-60 cells can produce markedly different results , which have been documented extensively over the last few years. We now report changes in electrical conductivity of Jurkat cells subjected to traditional electroporation pulses (50 ms pulse length) and ultrashort pulses (10 ns pulse length) using time domain dielectric spectroscopy (TDS). A single 10 ns, 150 kV/cm pulse did not noticeably alter suspension conductivity while a 50 ms, 2.12 kV/cm pulse with the same energy caused an appreciable conductivity rise. These results support the hypothesis that electroporation pulses primarily interact with the cell membrane and cause conductivity rises due to ion transport from the cell to the external media, while pulses with nanosecond duration primarily interact with the membranes of intracellular organelles. However, multiple ultrashort pulses have a cumulative effect on the plasma membrane, with five pulses causing a gradual rise in conductivity up to ten minutes post-pulsing.
Conference or Workshop Item
||Event Dates: Oct 4--8, 2004
|Venue - Dates:
||3rd International workshop on biological effect of EMFs, Greece, 2004-10-04 - 2004-10-08
||Jurkat cell, electrical conductivity, ultrashort electric pulse
||Electronics & Computer Science, EEE
||01 Dec 2004
||17 Apr 2017 22:18
|Further Information:||Google Scholar|
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