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Nanosecond electric pulses penetrate the nucleus and enhance speckle formation

Nanosecond electric pulses penetrate the nucleus and enhance speckle formation
Nanosecond electric pulses penetrate the nucleus and enhance speckle formation
Nanosecond electric pulses generate nanopores in the interior membranes of cells and modulate cellular functions. Here, we used confocal microscopy and flow cytometry to observe Smith antigen antibody (Y12) binding to nuclear speckles, known as small nuclear ribonucleoprotein particles (snRNPs) or intrachromatin granule clusters (IGCs), in Jurkat cells following one or five 10 ns, 150 kV/cm pulses. Using confocal microscopy and flow cytometry, we observed changes in nuclear speckle labeling that suggested a disruption of pre-messenger RNA splicing mechanisms. Pulse exposure increased the nuclear speckled substructures by 2.5-fold above basal levels while the propidium iodide (PI) uptake in pulsed cells was unchanged. The resulting nuclear speckle changes were also cell cycle dependent. These findings suggest that 10 ns pulses directly influenced nuclear processes, such as the changes in the nuclear RNA–protein complexes.
Nucleus, Nuclear speckles, Small nuclear ribonucleoprotein particles/intrachromatin granule clusters, Intracellular electromanipulation, Nanosecond electric pulses
0006-291X
220-225
Chen, N
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Garner, A L
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Chen, G
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Jing, Y
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Deng, Y
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Swanson, R J
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Kolb, J F
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Beebe, S J
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Joshi, R P
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Schoenbach, K H
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Chen, N
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Garner, A L
9b6674a0-6493-473c-90dc-1d4148a9782c
Chen, G
3de45a9c-6c9a-4bcb-90c3-d7e26be21819
Jing, Y
7e160060-7d1a-4963-9676-29e87c2c3105
Deng, Y
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Swanson, R J
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Kolb, J F
62c39789-61d5-403a-94e9-c6d1d4774766
Beebe, S J
ecdd6d6a-c270-45c0-a0bc-2f5c25c44cf7
Joshi, R P
c99508b0-5ec1-4a76-bfb9-7e7367aebd1b
Schoenbach, K H
9ba626fa-421b-47a0-8276-6825ccd99da6

Chen, N, Garner, A L, Chen, G, Jing, Y, Deng, Y, Swanson, R J, Kolb, J F, Beebe, S J, Joshi, R P and Schoenbach, K H (2007) Nanosecond electric pulses penetrate the nucleus and enhance speckle formation. Biochemical and Biophysical Research Communications, 364, 220-225.

Record type: Article

Abstract

Nanosecond electric pulses generate nanopores in the interior membranes of cells and modulate cellular functions. Here, we used confocal microscopy and flow cytometry to observe Smith antigen antibody (Y12) binding to nuclear speckles, known as small nuclear ribonucleoprotein particles (snRNPs) or intrachromatin granule clusters (IGCs), in Jurkat cells following one or five 10 ns, 150 kV/cm pulses. Using confocal microscopy and flow cytometry, we observed changes in nuclear speckle labeling that suggested a disruption of pre-messenger RNA splicing mechanisms. Pulse exposure increased the nuclear speckled substructures by 2.5-fold above basal levels while the propidium iodide (PI) uptake in pulsed cells was unchanged. The resulting nuclear speckle changes were also cell cycle dependent. These findings suggest that 10 ns pulses directly influenced nuclear processes, such as the changes in the nuclear RNA–protein complexes.

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Published date: December 2007
Keywords: Nucleus, Nuclear speckles, Small nuclear ribonucleoprotein particles/intrachromatin granule clusters, Intracellular electromanipulation, Nanosecond electric pulses
Organisations: Electronics & Computer Science, EEE

Identifiers

Local EPrints ID: 264761
URI: https://eprints.soton.ac.uk/id/eprint/264761
ISSN: 0006-291X
PURE UUID: d26af1a4-3061-4331-98ea-ad81ca394ba8

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Date deposited: 30 Oct 2007
Last modified: 30 Jul 2019 18:59

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