Design and Construction of a Programmable Electroporation system for Biological Applications
Design and Construction of a Programmable Electroporation system for Biological Applications
Studies into electroporation have grown rapidly in biotechnology and medicine in recent years. This paper presents the design and construction of a low cost programmable electroporation system for biological applications. The system consists of a control module, a pulse generation circuit and a high voltage switch using a power MOSFET. The programmable electroporation has been designed, developed and tested. Using a standard commercial electroporation cuvette, it is possible to generate electric fields of 100 to 1000V/cm with programmed pulse lengths of 10?sec to 20msec. The system was evaluated with Hela cells and propidium dye to evaluate transfection rates under a variety of electroporation conditions. Initial results showed that the electroporation system achieved a peak cell transfection efficiency of 48.74% at 600V/cm with pulse lengths of 10 ms.
234-238
Rodamporn, Somphop
e06728a0-30ec-43bc-a95c-677265248eef
Beeby, Steve
ba565001-2812-4300-89f1-fe5a437ecb0d
Harris, Nick
237cfdbd-86e4-4025-869c-c85136f14dfd
Brown, Andrew
5c19e523-65ec-499b-9e7c-91522017d7e0
Chad, John
2b52a3e0-a9e1-4988-996b-132e95ba38bd
December 2007
Rodamporn, Somphop
e06728a0-30ec-43bc-a95c-677265248eef
Beeby, Steve
ba565001-2812-4300-89f1-fe5a437ecb0d
Harris, Nick
237cfdbd-86e4-4025-869c-c85136f14dfd
Brown, Andrew
5c19e523-65ec-499b-9e7c-91522017d7e0
Chad, John
2b52a3e0-a9e1-4988-996b-132e95ba38bd
Rodamporn, Somphop, Beeby, Steve, Harris, Nick, Brown, Andrew and Chad, John
(2007)
Design and Construction of a Programmable Electroporation system for Biological Applications.
The 1st Symposium Thai Biomedical Engineering, Thailand.
18 - 19 Dec 2007.
.
Record type:
Conference or Workshop Item
(Other)
Abstract
Studies into electroporation have grown rapidly in biotechnology and medicine in recent years. This paper presents the design and construction of a low cost programmable electroporation system for biological applications. The system consists of a control module, a pulse generation circuit and a high voltage switch using a power MOSFET. The programmable electroporation has been designed, developed and tested. Using a standard commercial electroporation cuvette, it is possible to generate electric fields of 100 to 1000V/cm with programmed pulse lengths of 10?sec to 20msec. The system was evaluated with Hela cells and propidium dye to evaluate transfection rates under a variety of electroporation conditions. Initial results showed that the electroporation system achieved a peak cell transfection efficiency of 48.74% at 600V/cm with pulse lengths of 10 ms.
More information
Published date: December 2007
Additional Information:
Event Dates: 18-19 December 2007
Venue - Dates:
The 1st Symposium Thai Biomedical Engineering, Thailand, 2007-12-18 - 2007-12-19
Organisations:
EEE
Identifiers
Local EPrints ID: 265013
URI: http://eprints.soton.ac.uk/id/eprint/265013
PURE UUID: d08e010c-b44c-47b2-89e2-7337198a279e
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Date deposited: 07 Jan 2008 14:16
Last modified: 07 Dec 2024 02:35
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Contributors
Author:
Somphop Rodamporn
Author:
Steve Beeby
Author:
Nick Harris
Author:
Andrew Brown
Author:
John Chad
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