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Design and Construction of a Programmable Electroporation system for Biological Applications

Rodamporn, Somphop, Beeby, Steve, Harris, Nick, Brown, Andrew and Chad, John (2007) Design and Construction of a Programmable Electroporation system for Biological Applications At The 1st Symposium Thai Biomedical Engineering, Thailand. 18 - 19 Dec 2007. , pp. 234-238.

Record type: Conference or Workshop Item (Other)


Studies into electroporation have grown rapidly in biotechnology and medicine in recent years. This paper presents the design and construction of a low cost programmable electroporation system for biological applications. The system consists of a control module, a pulse generation circuit and a high voltage switch using a power MOSFET. The programmable electroporation has been designed, developed and tested. Using a standard commercial electroporation cuvette, it is possible to generate electric fields of 100 to 1000V/cm with programmed pulse lengths of 10?sec to 20msec. The system was evaluated with Hela cells and propidium dye to evaluate transfection rates under a variety of electroporation conditions. Initial results showed that the electroporation system achieved a peak cell transfection efficiency of 48.74% at 600V/cm with pulse lengths of 10 ms.

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Published date: December 2007
Additional Information: Event Dates: 18-19 December 2007
Venue - Dates: The 1st Symposium Thai Biomedical Engineering, Thailand, 2007-12-18 - 2007-12-19
Organisations: EEE


Local EPrints ID: 265013
PURE UUID: d08e010c-b44c-47b2-89e2-7337198a279e
ORCID for Steve Beeby: ORCID iD
ORCID for Nick Harris: ORCID iD

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Date deposited: 07 Jan 2008 14:16
Last modified: 18 Jul 2017 07:30

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Author: Somphop Rodamporn
Author: Steve Beeby ORCID iD
Author: Nick Harris ORCID iD
Author: Andrew Brown
Author: John Chad

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