The University of Southampton
University of Southampton Institutional Repository

Design and Construction of a Programmable Electroporation system for Biological Applications

Design and Construction of a Programmable Electroporation system for Biological Applications
Design and Construction of a Programmable Electroporation system for Biological Applications
Studies into electroporation have grown rapidly in biotechnology and medicine in recent years. This paper presents the design and construction of a low cost programmable electroporation system for biological applications. The system consists of a control module, a pulse generation circuit and a high voltage switch using a power MOSFET. The programmable electroporation has been designed, developed and tested. Using a standard commercial electroporation cuvette, it is possible to generate electric fields of 100 to 1000V/cm with programmed pulse lengths of 10?sec to 20msec. The system was evaluated with Hela cells and propidium dye to evaluate transfection rates under a variety of electroporation conditions. Initial results showed that the electroporation system achieved a peak cell transfection efficiency of 48.74% at 600V/cm with pulse lengths of 10 ms.
234-238
Rodamporn, Somphop
e06728a0-30ec-43bc-a95c-677265248eef
Beeby, Steve
ba565001-2812-4300-89f1-fe5a437ecb0d
Harris, Nick
237cfdbd-86e4-4025-869c-c85136f14dfd
Brown, Andrew
5c19e523-65ec-499b-9e7c-91522017d7e0
Chad, John
2b52a3e0-a9e1-4988-996b-132e95ba38bd
Rodamporn, Somphop
e06728a0-30ec-43bc-a95c-677265248eef
Beeby, Steve
ba565001-2812-4300-89f1-fe5a437ecb0d
Harris, Nick
237cfdbd-86e4-4025-869c-c85136f14dfd
Brown, Andrew
5c19e523-65ec-499b-9e7c-91522017d7e0
Chad, John
2b52a3e0-a9e1-4988-996b-132e95ba38bd

Rodamporn, Somphop, Beeby, Steve, Harris, Nick, Brown, Andrew and Chad, John (2007) Design and Construction of a Programmable Electroporation system for Biological Applications At The 1st Symposium Thai Biomedical Engineering, Thailand. 18 - 19 Dec 2007. , pp. 234-238.

Record type: Conference or Workshop Item (Other)

Abstract

Studies into electroporation have grown rapidly in biotechnology and medicine in recent years. This paper presents the design and construction of a low cost programmable electroporation system for biological applications. The system consists of a control module, a pulse generation circuit and a high voltage switch using a power MOSFET. The programmable electroporation has been designed, developed and tested. Using a standard commercial electroporation cuvette, it is possible to generate electric fields of 100 to 1000V/cm with programmed pulse lengths of 10?sec to 20msec. The system was evaluated with Hela cells and propidium dye to evaluate transfection rates under a variety of electroporation conditions. Initial results showed that the electroporation system achieved a peak cell transfection efficiency of 48.74% at 600V/cm with pulse lengths of 10 ms.

PDF 53.pdf - Other
Download (356kB)

More information

Published date: December 2007
Additional Information: Event Dates: 18-19 December 2007
Venue - Dates: The 1st Symposium Thai Biomedical Engineering, Thailand, 2007-12-18 - 2007-12-19
Organisations: EEE

Identifiers

Local EPrints ID: 265013
URI: http://eprints.soton.ac.uk/id/eprint/265013
PURE UUID: d08e010c-b44c-47b2-89e2-7337198a279e
ORCID for Steve Beeby: ORCID iD orcid.org/0000-0002-0800-1759
ORCID for Nick Harris: ORCID iD orcid.org/0000-0003-4122-2219

Catalogue record

Date deposited: 07 Jan 2008 14:16
Last modified: 18 Jul 2017 07:30

Export record

Contributors

Author: Somphop Rodamporn
Author: Steve Beeby ORCID iD
Author: Nick Harris ORCID iD
Author: Andrew Brown
Author: John Chad

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×