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Lipid layers on polyelectrolyte multilayer supports

Lipid layers on polyelectrolyte multilayer supports
Lipid layers on polyelectrolyte multilayer supports
The mechanism of formation of supported lipid layers from phosphatidylcholine and phosphatidylserine vesicles in solution on polyelectrolyte multilayers was studied by a variety of experimental techniques. The interaction of zwitterionic and acidic lipid vesicles, as well as their mixtures, with polyelectrolyte supports was followed in real time by micro-gravimetry. The fabricated lipid–polyelectrolyte composite structures on top of multilayer coated colloidal particles were characterized by flow cytometry and imaging techniques. Lipid diffusion over the macroscopic scale was quantified by fluorescence recovery after photobleaching, and the diffusion was related to layer connectivity. The phospholipid–polyelectrolyte binding mechanism was investigated by infrared spectroscopy. A strong interaction of polyelectrolyte primary amino groups with phosphate and carboxyl groups of the phospholipids, leading to dehydration, was observed. Long-range electrostatic attraction was proven to be essential for vesicle spreading and rupture. Fusion of lipid patches into a homogeneous bilayer required lateral mobility of the lipids on the polyelectrolyte support. The binding of amino groups to the phosphate group of the zwitterionic lipids was too weak to induce vesicle spreading, but sufficient for strong adsorption. Only the mixture of phosphatidylcholine and phosphatidylserine resulted in the spontaneous formation of bilayers on polyelectrolyte multilayers. The adsorption of phospholipids onto multilayers displaying quarternary ammonium polymers produced a novel 3D lipid polyelectrolyte structure on colloidal particles.



1744-683X
2245-2258
Fischlechner, Martin
b3930129-0775-4c05-81c7-475934df97ee
Zaulig, Markus
087fd6bd-dc0c-4615-a87d-438f5d0c0b0f
Meyer, Stefan
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Estrela-Lopis, Irina
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Cuéllar, Luis
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Irigoyen, Joseba
0c673d8e-976f-4672-a219-c0826d7746a5
Pescador, Paula
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Brumen, Milan
2bed1abb-2703-4585-8485-eb07660c642a
Messner, Paul
87b9cf4f-96d0-4b0f-ad73-2939c8ced1e1
Moya, Sergio
b6034c12-474d-4a3d-a25b-9868b314ce40
Donath, Edwin
a1baee66-cc02-4a15-8c0d-732e844b8a8f
Fischlechner, Martin
b3930129-0775-4c05-81c7-475934df97ee
Zaulig, Markus
087fd6bd-dc0c-4615-a87d-438f5d0c0b0f
Meyer, Stefan
a2525a9b-600d-4aa3-80ff-dd6c0eb3d4d0
Estrela-Lopis, Irina
604fcdf7-7503-4410-845d-38a8d3b2104e
Cuéllar, Luis
ab49b713-58a7-4bd6-8692-a5093c12ea40
Irigoyen, Joseba
0c673d8e-976f-4672-a219-c0826d7746a5
Pescador, Paula
6ab83b78-a030-4a96-89c5-625ae2df31d1
Brumen, Milan
2bed1abb-2703-4585-8485-eb07660c642a
Messner, Paul
87b9cf4f-96d0-4b0f-ad73-2939c8ced1e1
Moya, Sergio
b6034c12-474d-4a3d-a25b-9868b314ce40
Donath, Edwin
a1baee66-cc02-4a15-8c0d-732e844b8a8f

Fischlechner, Martin, Zaulig, Markus, Meyer, Stefan, Estrela-Lopis, Irina, Cuéllar, Luis, Irigoyen, Joseba, Pescador, Paula, Brumen, Milan, Messner, Paul, Moya, Sergio and Donath, Edwin (2008) Lipid layers on polyelectrolyte multilayer supports. Soft Matter, 4 (11), 2245-2258. (doi:10.1039/b805754k).

Record type: Article

Abstract

The mechanism of formation of supported lipid layers from phosphatidylcholine and phosphatidylserine vesicles in solution on polyelectrolyte multilayers was studied by a variety of experimental techniques. The interaction of zwitterionic and acidic lipid vesicles, as well as their mixtures, with polyelectrolyte supports was followed in real time by micro-gravimetry. The fabricated lipid–polyelectrolyte composite structures on top of multilayer coated colloidal particles were characterized by flow cytometry and imaging techniques. Lipid diffusion over the macroscopic scale was quantified by fluorescence recovery after photobleaching, and the diffusion was related to layer connectivity. The phospholipid–polyelectrolyte binding mechanism was investigated by infrared spectroscopy. A strong interaction of polyelectrolyte primary amino groups with phosphate and carboxyl groups of the phospholipids, leading to dehydration, was observed. Long-range electrostatic attraction was proven to be essential for vesicle spreading and rupture. Fusion of lipid patches into a homogeneous bilayer required lateral mobility of the lipids on the polyelectrolyte support. The binding of amino groups to the phosphate group of the zwitterionic lipids was too weak to induce vesicle spreading, but sufficient for strong adsorption. Only the mixture of phosphatidylcholine and phosphatidylserine resulted in the spontaneous formation of bilayers on polyelectrolyte multilayers. The adsorption of phospholipids onto multilayers displaying quarternary ammonium polymers produced a novel 3D lipid polyelectrolyte structure on colloidal particles.



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Published date: 18 August 2008
Organisations: Chemistry

Identifiers

Local EPrints ID: 340193
URI: http://eprints.soton.ac.uk/id/eprint/340193
ISSN: 1744-683X
PURE UUID: 060aa6fc-237b-45c2-acaa-ee4c8fdb2c60

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Date deposited: 14 Jun 2012 14:14
Last modified: 09 Dec 2019 20:06

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Contributors

Author: Martin Fischlechner
Author: Markus Zaulig
Author: Stefan Meyer
Author: Irina Estrela-Lopis
Author: Luis Cuéllar
Author: Joseba Irigoyen
Author: Paula Pescador
Author: Milan Brumen
Author: Paul Messner
Author: Sergio Moya
Author: Edwin Donath

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