The University of Southampton
University of Southampton Institutional Repository

A nanotechnology approach to DNA analysis

A nanotechnology approach to DNA analysis
A nanotechnology approach to DNA analysis
This thesis describes the investigation of quantum dots and nano-structured metallic films for use in single genomic DNA analysis. The fluorescence of continuously illuminated core/shell CdSe/ZnS quantum dots (QDs) under various atmospheric conditions is investigated experimentally. Initial enhancement in fluorescence intensity is observed followed by degradation; both are highly dependent on the atmospheric conditions. Following a series of studies theories are put forward to explain these observations.

Solution mixtures of DNA strands and QDs are imaged with Atomic Force Microscopy and Fluorescence Microscopy to investigate the binding properties of DNA strands with QDs.

Gold nanovoids are fabricated for use as a substrate to provide localised enhanced fluorescence intensity of fluorescently labelled regions of DNA strands stretched and located over nanovoids as a result of resonant coupling with localised surface plasmon polariton modes. The energy and electric field distribution of localised surface plasmon polaritons is considered for various void geometries for use with fluorescently labelled DNA. The experimental fluorescence intensity profile along fluorescently labelled DNA strands stretched over glass, electrochemically grown gold and gold nanovoid substrates is compared and the fluorescence lifetime is measured. Short fluorescence lifetimes and increased intensities over gold nanovoids and a constant lifetime over glass are observed.

The results of these biophysical studies are discussed with a view for application as methods for distinguishing different DNA sequences on the nanoscale.
University of Southampton
Pechstedt, Katrin
5e108ee2-e41b-4d05-8e18-ead38d018d5f
Pechstedt, Katrin
5e108ee2-e41b-4d05-8e18-ead38d018d5f
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f

Pechstedt, Katrin (2011) A nanotechnology approach to DNA analysis. University of Southampton, Faculty of Physical and Applied Sciences, Doctoral Thesis, 365pp.

Record type: Thesis (Doctoral)

Abstract

This thesis describes the investigation of quantum dots and nano-structured metallic films for use in single genomic DNA analysis. The fluorescence of continuously illuminated core/shell CdSe/ZnS quantum dots (QDs) under various atmospheric conditions is investigated experimentally. Initial enhancement in fluorescence intensity is observed followed by degradation; both are highly dependent on the atmospheric conditions. Following a series of studies theories are put forward to explain these observations.

Solution mixtures of DNA strands and QDs are imaged with Atomic Force Microscopy and Fluorescence Microscopy to investigate the binding properties of DNA strands with QDs.

Gold nanovoids are fabricated for use as a substrate to provide localised enhanced fluorescence intensity of fluorescently labelled regions of DNA strands stretched and located over nanovoids as a result of resonant coupling with localised surface plasmon polariton modes. The energy and electric field distribution of localised surface plasmon polaritons is considered for various void geometries for use with fluorescently labelled DNA. The experimental fluorescence intensity profile along fluorescently labelled DNA strands stretched over glass, electrochemically grown gold and gold nanovoid substrates is compared and the fluorescence lifetime is measured. Short fluorescence lifetimes and increased intensities over gold nanovoids and a constant lifetime over glass are observed.

The results of these biophysical studies are discussed with a view for application as methods for distinguishing different DNA sequences on the nanoscale.

Text
KPechstedt_PhD_Thesis_2011.pdf - Version of Record
Available under License University of Southampton Thesis Licence.
Download (10MB)

More information

Published date: May 2011
Organisations: University of Southampton, Optoelectronics Research Centre

Identifiers

Local EPrints ID: 340250
URI: http://eprints.soton.ac.uk/id/eprint/340250
PURE UUID: 154269bd-67b1-4cc1-9962-a08f8a8a77ac

Catalogue record

Date deposited: 18 Sep 2012 15:59
Last modified: 29 Aug 2019 05:41

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×