The University of Southampton
University of Southampton Institutional Repository

Development of biocompatible polymers for ocular applications

Development of biocompatible polymers for ocular applications
Development of biocompatible polymers for ocular applications
Age-related macular degeneration (AMD) is the largest cause of blindness for those over 65 in the developed world. There is currently no treatment for the retinal cellular loss associated with the disease. One potential therapy is to implant retinal stem cells into the eye using a biodegradable polymer scaffold. Blends of the biodegradable polymers, poly(L-lactic acid) (PLLA) and poly(D,L-lactic-co-glycolic acid) (PLGA) have been formulated into microspheres. The influence of changing processing parameters on the size and morphology of the microspheres has been studied. A human retinal pigment epithelial (APRE-19) cell line was shown to adhere, survive and proliferate on the surface of the microspheres in vitro. Assays have demonstrated that the nature of the blend influenced cell behaviour.
Transplantation of retinal pigment epithelial (RPE) cells on a supportive matrix has also been investigated as a therapy for AMD. In view of AMD related pathology of the native RPE support, Bruch’s membrane (BM), transplanted RPE cells require a scaffold to reside on. Copolymers based on methyl methacrylate (MMA) and poly(ethylene glycol) methacrylate (PEGM) have been synthesised and chemically modified at the PEG terminus. These polymers were subsequently manufactured into a fibrous scaffold using an electrospinning technique and investigated as an artificial BM. RPE cells were shown to attach and proliferate successfully on the surface of the fibrous scaffold in vitro. Cell adhesion was significantly enhanced on scaffolds with the PEG chain terminus modification. Significantly less apoptotic cell death was also observed on these surfaces. The diffusion properties of these artificial membranes have also been investigated. In addition, the novel gelation of the produced copolymers under certain conditions has been studied
Treharne, Andrew J
5ea8fb01-1e91-4c18-bd0b-fff03383e0d8
Treharne, Andrew J
5ea8fb01-1e91-4c18-bd0b-fff03383e0d8
Grossel, Martin
403bf3ff-6364-44e9-ab46-52d84c6f0d56

Treharne, Andrew J (2012) Development of biocompatible polymers for ocular applications. University of Southampton, Chemistry, Doctoral Thesis, 245pp.

Record type: Thesis (Doctoral)

Abstract

Age-related macular degeneration (AMD) is the largest cause of blindness for those over 65 in the developed world. There is currently no treatment for the retinal cellular loss associated with the disease. One potential therapy is to implant retinal stem cells into the eye using a biodegradable polymer scaffold. Blends of the biodegradable polymers, poly(L-lactic acid) (PLLA) and poly(D,L-lactic-co-glycolic acid) (PLGA) have been formulated into microspheres. The influence of changing processing parameters on the size and morphology of the microspheres has been studied. A human retinal pigment epithelial (APRE-19) cell line was shown to adhere, survive and proliferate on the surface of the microspheres in vitro. Assays have demonstrated that the nature of the blend influenced cell behaviour.
Transplantation of retinal pigment epithelial (RPE) cells on a supportive matrix has also been investigated as a therapy for AMD. In view of AMD related pathology of the native RPE support, Bruch’s membrane (BM), transplanted RPE cells require a scaffold to reside on. Copolymers based on methyl methacrylate (MMA) and poly(ethylene glycol) methacrylate (PEGM) have been synthesised and chemically modified at the PEG terminus. These polymers were subsequently manufactured into a fibrous scaffold using an electrospinning technique and investigated as an artificial BM. RPE cells were shown to attach and proliferate successfully on the surface of the fibrous scaffold in vitro. Cell adhesion was significantly enhanced on scaffolds with the PEG chain terminus modification. Significantly less apoptotic cell death was also observed on these surfaces. The diffusion properties of these artificial membranes have also been investigated. In addition, the novel gelation of the produced copolymers under certain conditions has been studied

Text
Andrew_John_Treharne_PhD_thesis.pdf - Version of Record
Available under License University of Southampton Thesis Licence.
Download (5MB)

More information

Published date: 31 August 2012
Organisations: University of Southampton, Chemistry

Identifiers

Local EPrints ID: 344289
URI: http://eprints.soton.ac.uk/id/eprint/344289
PURE UUID: 77a41492-519e-4861-9e35-92d270106b89
ORCID for Martin Grossel: ORCID iD orcid.org/0000-0001-7469-6854

Catalogue record

Date deposited: 18 Feb 2013 16:22
Last modified: 29 Jan 2019 05:01

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×