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A large geometric distortion in the first photointermediate of rhodopsin, determined by double-quantum solid-state NMR

A large geometric distortion in the first photointermediate of rhodopsin, determined by double-quantum solid-state NMR
A large geometric distortion in the first photointermediate of rhodopsin, determined by double-quantum solid-state NMR
Double-quantum magic-angle-spinning NMR experiments were performed on 11,12-C-13(2)-retinylidene-rhodopsin under illumination at low temperature, in order to characterize torsional angle changes at the C11-C12 photoisomerization site. The sample was illuminated in the NMR rotor at low temperature (similar to 120 K) in order to trap the primary photointermediate, bathorhodopsin. The NMR data are consistent with a strong torsional twist of the HCCH moiety at the isomerization site. Although the HCCH torsional twist was determined to be at least 40A degrees, it was not possible to quantify it more closely. The presence of a strong twist is in agreement with previous Raman observations. The energetic implications of this geometric distortion are discussed.
0925-2738
247-256
Concistrè, Maria
4d926227-2c26-489b-ac6c-ccc1354c68db
Johannessen, Ole G.
799ccc8c-a2e7-4305-a03a-2dc9f42564ef
McLean, Neville
3250113c-28d4-499b-8282-e54c3d8a9862
Bovee-Geurts, Petra H. M.
46c26df2-56a8-42f8-a154-d953e53e1fdf
Brown, Richard C. D.
21ce697a-7c3a-480e-919f-429a3d8550f5
DeGrip, Willem J.
3fe658dc-e4cd-4d05-9771-417fd462ae72
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3
Concistrè, Maria
4d926227-2c26-489b-ac6c-ccc1354c68db
Johannessen, Ole G.
799ccc8c-a2e7-4305-a03a-2dc9f42564ef
McLean, Neville
3250113c-28d4-499b-8282-e54c3d8a9862
Bovee-Geurts, Petra H. M.
46c26df2-56a8-42f8-a154-d953e53e1fdf
Brown, Richard C. D.
21ce697a-7c3a-480e-919f-429a3d8550f5
DeGrip, Willem J.
3fe658dc-e4cd-4d05-9771-417fd462ae72
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3

Concistrè, Maria, Johannessen, Ole G., McLean, Neville, Bovee-Geurts, Petra H. M., Brown, Richard C. D., DeGrip, Willem J. and Levitt, Malcolm H. (2012) A large geometric distortion in the first photointermediate of rhodopsin, determined by double-quantum solid-state NMR. Journal of Biomolecular NMR, 53 (3), 247-256. (doi:10.1007/s10858-012-9635-4). (PMID:22639195)

Record type: Article

Abstract

Double-quantum magic-angle-spinning NMR experiments were performed on 11,12-C-13(2)-retinylidene-rhodopsin under illumination at low temperature, in order to characterize torsional angle changes at the C11-C12 photoisomerization site. The sample was illuminated in the NMR rotor at low temperature (similar to 120 K) in order to trap the primary photointermediate, bathorhodopsin. The NMR data are consistent with a strong torsional twist of the HCCH moiety at the isomerization site. Although the HCCH torsional twist was determined to be at least 40A degrees, it was not possible to quantify it more closely. The presence of a strong twist is in agreement with previous Raman observations. The energetic implications of this geometric distortion are discussed.

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e-pub ahead of print date: 26 May 2012
Published date: July 2012
Organisations: Magnetic Resonance

Identifiers

Local EPrints ID: 347835
URI: http://eprints.soton.ac.uk/id/eprint/347835
ISSN: 0925-2738
PURE UUID: d8de2e83-a0b2-46ff-a0cf-1b3ecd739811
ORCID for Richard C. D. Brown: ORCID iD orcid.org/0000-0003-0156-7087
ORCID for Malcolm H. Levitt: ORCID iD orcid.org/0000-0001-9878-1180

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Date deposited: 31 Jan 2013 13:37
Last modified: 15 Mar 2024 03:08

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Contributors

Author: Maria Concistrè
Author: Ole G. Johannessen
Author: Neville McLean
Author: Petra H. M. Bovee-Geurts
Author: Willem J. DeGrip

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