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Reversible ligation of programmed DNA-gold nanoparticle assemblies

Reversible ligation of programmed DNA-gold nanoparticle assemblies
Reversible ligation of programmed DNA-gold nanoparticle assemblies
We demonstrate a new method to reversibly cross-link DNA-nanoparticle dimers, trimers, and tetramers using light as an external stimulus. A DNA interstrand photo-cross-linking reaction is possible via ligation of a cyano-vinyl carbazole nucleoside with an opposite thymine when irradiated at 365 nm. This reaction results in nanoparticle assemblies that are not susceptible to DNA dehybridization conditions. The chemical bond between the two complementary DNA strands can be reversibly broken upon light irradiation at 312 nm. This is the first example of reversible ligation in DNA-nanoparticle assemblies using light and enables new developments in the field of programmed nanoparticle organization.
0002-7863
9242-9245
Harimech, Pascal K.
b8962aad-d718-4f1b-8128-0c7c4c222069
Gerrard, Simon R.
4ed7b304-42c2-497a-b00d-5ac8a1bedd24
El-Sagheer, Afaf H.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Harimech, Pascal K.
b8962aad-d718-4f1b-8128-0c7c4c222069
Gerrard, Simon R.
4ed7b304-42c2-497a-b00d-5ac8a1bedd24
El-Sagheer, Afaf H.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7

Harimech, Pascal K., Gerrard, Simon R., El-Sagheer, Afaf H., Brown, Tom and Kanaras, Antonios G. (2015) Reversible ligation of programmed DNA-gold nanoparticle assemblies. Journal of the American Chemical Society, 137 (29), 9242-9245. (doi:10.1021/jacs.5b05683).

Record type: Article

Abstract

We demonstrate a new method to reversibly cross-link DNA-nanoparticle dimers, trimers, and tetramers using light as an external stimulus. A DNA interstrand photo-cross-linking reaction is possible via ligation of a cyano-vinyl carbazole nucleoside with an opposite thymine when irradiated at 365 nm. This reaction results in nanoparticle assemblies that are not susceptible to DNA dehybridization conditions. The chemical bond between the two complementary DNA strands can be reversibly broken upon light irradiation at 312 nm. This is the first example of reversible ligation in DNA-nanoparticle assemblies using light and enables new developments in the field of programmed nanoparticle organization.

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e-pub ahead of print date: 20 July 2015
Published date: 20 July 2015
Organisations: Chemistry, Quantum, Light & Matter Group

Identifiers

Local EPrints ID: 379565
URI: https://eprints.soton.ac.uk/id/eprint/379565
ISSN: 0002-7863
PURE UUID: bc488dd1-2795-437e-94e7-7dfcfe1212b5
ORCID for Antonios G. Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

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Date deposited: 05 Aug 2015 14:52
Last modified: 20 Jul 2019 00:51

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Contributors

Author: Pascal K. Harimech
Author: Simon R. Gerrard
Author: Afaf H. El-Sagheer
Author: Tom Brown

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