Effects of the addition of waste cooking oil on heavy crude oil biodegradation and microbial enhanced oil recovery using Pseudomonas sp. SWP- 4
Effects of the addition of waste cooking oil on heavy crude oil biodegradation and microbial enhanced oil recovery using Pseudomonas sp. SWP- 4
The present work aims to investigate the effects of the addition of waste cooking oil (WCO) on heavy crude oil biodegradation and microbial enhanced oil recovery (MEOR) using Pseudomonas sp. SWP-4. Growth kinetics show Pseudomonas sp. SWP-4 had a maximum dry cell weight of 1.73 g/L and cell-surface hydrophobicity of 62.4% against n-hexadecane when degraded the crude oil with the addition of WCO. The maximum rhamnolipid concentration was 6.87 g/L, and the culture broth exhibited a higher emulsification efficiency of 58.3% on n-hexadecane and reduced the surface tension of broth to 22.7 mN/m. Meanwhile, Pseudomonas sp. SWP-4 reduced the viscosity of crude oil from 26,300 mPa s to 550 mPa s (40 °C) and successfully degraded most of the n-alkanes. Furthermore, the fluidity of oil had been well improved after degradation. It can be concluded that not only could WCO stimulate the bacterial growth, but also it could enhance the crude oil degradation. Core displacement experiment demonstrates the efficiency of water flooding was just 5.8%, but the microbial flooding produced by Pseudomonas sp. SWP-4 with the addition of WCO effectively improved the oil recovery further with an additional oil recovery efficiency of 24.4%
waste cooking oil, biodegradation, microbial enhanced oil recovery, growth kinetics, viscosity, bioremediation
219-226
Lan, Guihong
0ae877a8-10a5-4c24-add4-c4c9c8f0ec43
Fan, Qiang
e3c612b3-bd58-42c9-ba1d-415d133aa566
Liu, Yongqiang
75adc6f8-aa83-484e-9e87-6c8442e344fa
Liu, Yu
7aa0a5b8-dca4-4f81-b6c9-7332b1beb25c
Liu, Yucheng
8434394e-bffc-4a23-a1b7-29dd6ca8e7fa
Yin, Xiaobo
122fe1d9-e108-4a42-821f-29f0a5906acb
Luo, Mina
5ec13361-a19e-40c5-bd27-88ddcfde7610
15 November 2015
Lan, Guihong
0ae877a8-10a5-4c24-add4-c4c9c8f0ec43
Fan, Qiang
e3c612b3-bd58-42c9-ba1d-415d133aa566
Liu, Yongqiang
75adc6f8-aa83-484e-9e87-6c8442e344fa
Liu, Yu
7aa0a5b8-dca4-4f81-b6c9-7332b1beb25c
Liu, Yucheng
8434394e-bffc-4a23-a1b7-29dd6ca8e7fa
Yin, Xiaobo
122fe1d9-e108-4a42-821f-29f0a5906acb
Luo, Mina
5ec13361-a19e-40c5-bd27-88ddcfde7610
Lan, Guihong, Fan, Qiang, Liu, Yongqiang, Liu, Yu, Liu, Yucheng, Yin, Xiaobo and Luo, Mina
(2015)
Effects of the addition of waste cooking oil on heavy crude oil biodegradation and microbial enhanced oil recovery using Pseudomonas sp. SWP- 4.
Biochemical Engineering Journal, 103, .
(doi:10.1016/j.bej.2015.08.004).
Abstract
The present work aims to investigate the effects of the addition of waste cooking oil (WCO) on heavy crude oil biodegradation and microbial enhanced oil recovery (MEOR) using Pseudomonas sp. SWP-4. Growth kinetics show Pseudomonas sp. SWP-4 had a maximum dry cell weight of 1.73 g/L and cell-surface hydrophobicity of 62.4% against n-hexadecane when degraded the crude oil with the addition of WCO. The maximum rhamnolipid concentration was 6.87 g/L, and the culture broth exhibited a higher emulsification efficiency of 58.3% on n-hexadecane and reduced the surface tension of broth to 22.7 mN/m. Meanwhile, Pseudomonas sp. SWP-4 reduced the viscosity of crude oil from 26,300 mPa s to 550 mPa s (40 °C) and successfully degraded most of the n-alkanes. Furthermore, the fluidity of oil had been well improved after degradation. It can be concluded that not only could WCO stimulate the bacterial growth, but also it could enhance the crude oil degradation. Core displacement experiment demonstrates the efficiency of water flooding was just 5.8%, but the microbial flooding produced by Pseudomonas sp. SWP-4 with the addition of WCO effectively improved the oil recovery further with an additional oil recovery efficiency of 24.4%
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Accepted/In Press date: 5 August 2015
Published date: 15 November 2015
Keywords:
waste cooking oil, biodegradation, microbial enhanced oil recovery, growth kinetics, viscosity, bioremediation
Organisations:
Water & Environmental Engineering Group
Identifiers
Local EPrints ID: 381373
URI: http://eprints.soton.ac.uk/id/eprint/381373
ISSN: 1369-703X
PURE UUID: 4a6e36e7-8a79-4eb1-8831-39efd17a5063
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Date deposited: 02 Oct 2015 10:36
Last modified: 15 Mar 2024 03:47
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Contributors
Author:
Guihong Lan
Author:
Qiang Fan
Author:
Yu Liu
Author:
Yucheng Liu
Author:
Xiaobo Yin
Author:
Mina Luo
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