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Dataset for low cost nanoribbon sensors for protein analysis in human serum using a miniature bead-based enzyme-linked immunosorbent assay

Dataset for low cost nanoribbon sensors for protein analysis in human serum using a miniature bead-based enzyme-linked immunosorbent assay
Dataset for low cost nanoribbon sensors for protein analysis in human serum using a miniature bead-based enzyme-linked immunosorbent assay
We describe a potentially low cost thin-film transistor (TFT) nanoribbon sensor for detection of the inflammatory biomarker C-reactive protein (CRP) in human serum via a miniature bead-based Enzyme-Linked Immunosorbent Assay (ELISA). The TFT sensor measures the reaction products from the ELISA via pH changes. The bead-based ELISA decouples the protein functionalization steps from the sensor surface, increasing the signal and simplifying the assay. The ability to directly sense proteins in human serum in this way overcomes the Debye length limitation associated with nanowire and nanoribbon biosensors. Compared to classically fabricated nanowires, the TFT nanoribbons are simple, extremely easy to fabricate and should therefore be much cheaper to manufacture. TFT nanoribbons, configured as pH sensors were used for quantitative detection of CRP spikes into human serum at concentrations as low as 1 ng/mL, which is ten thousand times better than needed for diagnostic purposes, providing the potential for applications that require very high sensitivity.
nanoribbon, protein analysis, human serum, electrical reading, pH change, ELISA
University of Southampton
HU, CHUNXIAO
4892b566-6809-42a8-8285-1c1e93aac730
Zeimpekis-Karakonstantinos, Ioannis
a2c354ec-3891-497c-adac-89b3a5d96af0
Sun, Kai
b7c648a3-7be8-4613-9d4d-1bf937fb487b
Anderson, Sally
bb9101ff-f8f1-432a-9dfb-8dc46e52b006
Ashburn, Peter
68cef6b7-205b-47aa-9efb-f1f09f5c1038
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
HU, CHUNXIAO
4892b566-6809-42a8-8285-1c1e93aac730
Zeimpekis-Karakonstantinos, Ioannis
a2c354ec-3891-497c-adac-89b3a5d96af0
Sun, Kai
b7c648a3-7be8-4613-9d4d-1bf937fb487b
Anderson, Sally
bb9101ff-f8f1-432a-9dfb-8dc46e52b006
Ashburn, Peter
68cef6b7-205b-47aa-9efb-f1f09f5c1038
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

HU, CHUNXIAO, Zeimpekis-Karakonstantinos, Ioannis, Sun, Kai, Anderson, Sally, Ashburn, Peter and Morgan, Hywel (2016) Dataset for low cost nanoribbon sensors for protein analysis in human serum using a miniature bead-based enzyme-linked immunosorbent assay. University of Southampton [Dataset]

Record type: Dataset

Abstract

We describe a potentially low cost thin-film transistor (TFT) nanoribbon sensor for detection of the inflammatory biomarker C-reactive protein (CRP) in human serum via a miniature bead-based Enzyme-Linked Immunosorbent Assay (ELISA). The TFT sensor measures the reaction products from the ELISA via pH changes. The bead-based ELISA decouples the protein functionalization steps from the sensor surface, increasing the signal and simplifying the assay. The ability to directly sense proteins in human serum in this way overcomes the Debye length limitation associated with nanowire and nanoribbon biosensors. Compared to classically fabricated nanowires, the TFT nanoribbons are simple, extremely easy to fabricate and should therefore be much cheaper to manufacture. TFT nanoribbons, configured as pH sensors were used for quantitative detection of CRP spikes into human serum at concentrations as low as 1 ng/mL, which is ten thousand times better than needed for diagnostic purposes, providing the potential for applications that require very high sensitivity.

Other
Data_20set.xlsx - Dataset
Available under License Creative Commons Attribution.
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More information

Published date: 2016
Keywords: nanoribbon, protein analysis, human serum, electrical reading, pH change, ELISA
Organisations: Nanoelectronics and Nanotechnology, ORC Research, Electronics & Computer Science
Projects:
Low cost nanowire diagnostic platform
Funded by: UNSPECIFIED (EP/K502327/1)
31 October 2012 to 30 October 2015

Identifiers

Local EPrints ID: 386454
URI: https://eprints.soton.ac.uk/id/eprint/386454
PURE UUID: f358cd4b-01d4-47ad-bca1-10164cd1e650
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 06 Apr 2016 12:54
Last modified: 06 Jun 2018 12:45

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Contributors

Creator: CHUNXIAO HU
Creator: Ioannis Zeimpekis-Karakonstantinos
Creator: Kai Sun
Creator: Sally Anderson
Creator: Peter Ashburn
Creator: Hywel Morgan ORCID iD

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