The University of Southampton
University of Southampton Institutional Repository

DATASET - Integrated optical waveguides and inertial focussing microfluidics in silica for microflow cytometry applications

DATASET - Integrated optical waveguides and inertial focussing microfluidics in silica for microflow cytometry applications
DATASET - Integrated optical waveguides and inertial focussing microfluidics in silica for microflow cytometry applications
A platform for microflow cytometry is presented comprising monolithically integrated waveguides and deep microfluidics in a rugged silica chip. Integrated waveguides deliver excitation light to an etched microfluidic channel and also collect transmitted light. The fluidics are designed to employ inertial focusing to reduce signal variation by bringing the flowing beads onto the same plane as the excitation light beam. A fabrication process is described which exploits microelectronics mass production techniques including: sputtering, ICP etching and PECVD. Test devices were fabricated and the effectiveness of inertial focusing of 5.6 µm fluorescent beads was studied showing lateral and vertical confinement of flowing beads within the microfluidic channel. The fluorescence signals from flowing calibration beads were quantified and a basic analysis of beads used in an immunoassay for TNF. was performed. Finally the potential of this type of device for measuring the variation in optical transmission from input to output waveguide as beads flowed through the beam was evaluated.Paper published in 'Integrated optical waveguides and inertial focussing microfluidics in silica for microflow cytometry applications'
University of Southampton
Butement, Jonathan
24ec84ac-7e21-4f66-a2de-68b81f54da63
Hunt, Hamish
b58afe40-9102-4a47-8d81-1fdd5463f4fe
Rowe, David
a0e0fe82-5e29-42b8-b370-5236a722f015
Sessions, Neil
ee737092-56b4-403e-a2f9-764e07e42625
Clark, Owain
ec13bf95-1f01-4f7b-98f7-5b87f5910d7a
Hua, Ping
92fa76e2-970b-45f5-a459-d9f95e735303
Ganapathy, Senthil
a867686e-0535-46cc-ad85-c2342086b25b
Chad, John
d220e55e-3c13-4d1d-ae9a-1cfae8ccfbe1
Wilkinson, James
73483cf3-d9f2-4688-9b09-1c84257884ca
Butement, Jonathan
24ec84ac-7e21-4f66-a2de-68b81f54da63
Hunt, Hamish
b58afe40-9102-4a47-8d81-1fdd5463f4fe
Rowe, David
a0e0fe82-5e29-42b8-b370-5236a722f015
Sessions, Neil
ee737092-56b4-403e-a2f9-764e07e42625
Clark, Owain
ec13bf95-1f01-4f7b-98f7-5b87f5910d7a
Hua, Ping
92fa76e2-970b-45f5-a459-d9f95e735303
Ganapathy, Senthil
a867686e-0535-46cc-ad85-c2342086b25b
Chad, John
d220e55e-3c13-4d1d-ae9a-1cfae8ccfbe1
Wilkinson, James
73483cf3-d9f2-4688-9b09-1c84257884ca

Butement, Jonathan, Hunt, Hamish, Rowe, David, Sessions, Neil, Clark, Owain, Hua, Ping, Ganapathy, Senthil, Chad, John and Wilkinson, James (2016) DATASET - Integrated optical waveguides and inertial focussing microfluidics in silica for microflow cytometry applications. University of Southampton [Dataset]

Record type: Dataset

Abstract

A platform for microflow cytometry is presented comprising monolithically integrated waveguides and deep microfluidics in a rugged silica chip. Integrated waveguides deliver excitation light to an etched microfluidic channel and also collect transmitted light. The fluidics are designed to employ inertial focusing to reduce signal variation by bringing the flowing beads onto the same plane as the excitation light beam. A fabrication process is described which exploits microelectronics mass production techniques including: sputtering, ICP etching and PECVD. Test devices were fabricated and the effectiveness of inertial focusing of 5.6 µm fluorescent beads was studied showing lateral and vertical confinement of flowing beads within the microfluidic channel. The fluorescence signals from flowing calibration beads were quantified and a basic analysis of beads used in an immunoassay for TNF. was performed. Finally the potential of this type of device for measuring the variation in optical transmission from input to output waveguide as beads flowed through the beam was evaluated.Paper published in 'Integrated optical waveguides and inertial focussing microfluidics in silica for microflow cytometry applications'

Image (TIFF)
Assay_154_pgml_1.tif - Image
Available under License Creative Commons Attribution.
Download (33MB)
Image (TIFF)
Assay_154_pgml_2.tif - Image
Available under License Creative Commons Attribution.
Download (33MB)
Image (TIFF)
Assay_154_pgml_3.tif - Image
Available under License Creative Commons Attribution.
Download (33MB)
Image (TIFF)
Assay_Calibrator_1.tif - Image
Available under License Creative Commons Attribution.
Download (33MB)
Image (TIFF)
Assay_Calibrator_2.tif - Image
Available under License Creative Commons Attribution.
Download (33MB)
Image (TIFF)
Assay_Calibrator_3.tif - Image
Available under License Creative Commons Attribution.
Download (33MB)
Other
Inertial_focussing_data.xlsx - Dataset
Available under License Creative Commons Attribution.
Download (10kB)
Image (TIFF)
Inertial_focusing_characterisation_0_2uls.tif - Image
Available under License Creative Commons Attribution.
Download (130MB)
Other
TNFalpha_immunoassay_data.xlsx - Dataset
Available under License Creative Commons Attribution.
Download (20kB)

Show all 9 downloads.

More information

Published date: 2016
Organisations: Optoelectronics Research Centre, Optoelectronics Research Centre, Electronics & Computer Science, Biomedicine, Faculty of Physical Sciences and Engineering, Photonic Systems Circuits & Sensors

Identifiers

Local EPrints ID: 391971
URI: http://eprints.soton.ac.uk/id/eprint/391971
PURE UUID: 9e9212b1-80ac-4683-8fef-76e07382685a
ORCID for David Rowe: ORCID iD orcid.org/0000-0002-1167-150X
ORCID for Senthil Ganapathy: ORCID iD orcid.org/0000-0002-2733-3273
ORCID for John Chad: ORCID iD orcid.org/0000-0001-6442-4281
ORCID for James Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 12 Apr 2016 15:08
Last modified: 06 Jun 2018 13:19

Export record

Contributors

Creator: Jonathan Butement
Creator: Hamish Hunt
Creator: David Rowe ORCID iD
Creator: Neil Sessions
Creator: Owain Clark
Creator: Ping Hua
Creator: John Chad ORCID iD
Creator: James Wilkinson ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×