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FACS-sorted putative oogonial stem cells from the ovary are neither DDX4-positive nor germ cells

FACS-sorted putative oogonial stem cells from the ovary are neither DDX4-positive nor germ cells
FACS-sorted putative oogonial stem cells from the ovary are neither DDX4-positive nor germ cells
Whether the adult mammalian ovary contains oogonial stem cells (OSCs) is controversial. They have been isolated by a live-cell sorting method using the germ cell marker DDX4, which has previously been assumed to be cytoplasmic, not surfacebound. Furthermore their stem cell and germ cell characteristics remain disputed. Here we show that although OSC-like cells can be isolated from the ovary using an antibody to DDX4, there is no good in silico modelling to support the existence of a surfacebound DDX4. Furthermore these cells when isolated were not expressing DDX4, and did not initially possess germline identity. Despite these unremarkable beginnings, they acquired some pre-meiotic markers in culture, including DDX4, but critically never expressed oocyte-specific markers, and furthermore were not immortal but died after a few months. Our results suggest that freshly isolated OSCs are not germ stem cells, and are not being isolated by their DDX4 expression. However it may be that culture induces some pre-meiotic markers. In summary the present study offers weight to the dogma that the adult ovary is populated by a fixed number of oocytes and that adult de novo production is a rare or insignificant event.
1-9
Zarate-Garcia, Larissa
0aaccd68-6d0e-4ece-9486-7597d1241d6d
Lane, Simon I.R.
8e80111f-5012-4950-a228-dfb8fb9df52d
Merriman-Jones, Julie A.
252e23f3-b63f-46ed-9904-7986642b4afa
Jones, Keith T.
73e8e2b5-cd67-4691-b1a9-4e7bc9066af4
Zarate-Garcia, Larissa
0aaccd68-6d0e-4ece-9486-7597d1241d6d
Lane, Simon I.R.
8e80111f-5012-4950-a228-dfb8fb9df52d
Merriman-Jones, Julie A.
252e23f3-b63f-46ed-9904-7986642b4afa
Jones, Keith T.
73e8e2b5-cd67-4691-b1a9-4e7bc9066af4

Zarate-Garcia, Larissa, Lane, Simon I.R., Merriman-Jones, Julie A. and Jones, Keith T. (2016) FACS-sorted putative oogonial stem cells from the ovary are neither DDX4-positive nor germ cells. Scientific Reports, 6, 1-9, [27991]. (doi:10.1038/srep27991).

Record type: Article

Abstract

Whether the adult mammalian ovary contains oogonial stem cells (OSCs) is controversial. They have been isolated by a live-cell sorting method using the germ cell marker DDX4, which has previously been assumed to be cytoplasmic, not surfacebound. Furthermore their stem cell and germ cell characteristics remain disputed. Here we show that although OSC-like cells can be isolated from the ovary using an antibody to DDX4, there is no good in silico modelling to support the existence of a surfacebound DDX4. Furthermore these cells when isolated were not expressing DDX4, and did not initially possess germline identity. Despite these unremarkable beginnings, they acquired some pre-meiotic markers in culture, including DDX4, but critically never expressed oocyte-specific markers, and furthermore were not immortal but died after a few months. Our results suggest that freshly isolated OSCs are not germ stem cells, and are not being isolated by their DDX4 expression. However it may be that culture induces some pre-meiotic markers. In summary the present study offers weight to the dogma that the adult ovary is populated by a fixed number of oocytes and that adult de novo production is a rare or insignificant event.

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srep27991 - Version of Record
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Accepted/In Press date: 26 May 2016
e-pub ahead of print date: 15 June 2016
Organisations: Biomedicine

Identifiers

Local EPrints ID: 396001
URI: http://eprints.soton.ac.uk/id/eprint/396001
PURE UUID: 26d07d79-1ef7-4167-97eb-2cad19f39d7d
ORCID for Simon I.R. Lane: ORCID iD orcid.org/0000-0002-8155-0981
ORCID for Keith T. Jones: ORCID iD orcid.org/0000-0002-0294-0851

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Date deposited: 02 Jun 2016 12:41
Last modified: 17 Dec 2019 01:37

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Contributors

Author: Larissa Zarate-Garcia
Author: Simon I.R. Lane ORCID iD
Author: Julie A. Merriman-Jones
Author: Keith T. Jones ORCID iD

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