RNA-stable isotope probing: from carbon flow within key microbiota to targeted transcriptomes
RNA-stable isotope probing: from carbon flow within key microbiota to targeted transcriptomes
Stable isotope probing of RNA has enthused researchers right from its first introduction in 2002. The concept of a labelling-based detection of process-targeted microbes independent of cellular replication or growth has allowed for a much more direct handle on functionally relevant microbiota than by labelling of other biomarkers. This has led to a widespread application of the technology, and breakthroughs in our understanding of carbon flow in natural microbiomes, autotrophic and heterotrophic physiologies, microbial food webs, host-microbe interactions and environmental biotechnology. Recent studies detecting labelled mRNA demonstrate that RNA-SIP is not limited to the analysis of rRNA, but is currently developing towards an approach for accessing targeted transcriptomes. In combination with next-generation sequencing and other methodological advances, RNA-SIP will continue to deliver invaluable insights into the functioning of microbial communities.
83-89
Lueders, Tillmann
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Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Bradford, Laura
165c4e5b-772c-4db9-b7c4-c81847e1e43d
Manefield, Mike
d778947a-0610-44bf-a8c5-67809bced6a1
October 2016
Lueders, Tillmann
5e98402c-730c-4662-909b-8b8c93bb16a4
Dumont, Marc
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Bradford, Laura
165c4e5b-772c-4db9-b7c4-c81847e1e43d
Manefield, Mike
d778947a-0610-44bf-a8c5-67809bced6a1
Lueders, Tillmann, Dumont, Marc, Bradford, Laura and Manefield, Mike
(2016)
RNA-stable isotope probing: from carbon flow within key microbiota to targeted transcriptomes.
[in special issue: Analytical Biotechnology]
Current Opinion in Biotechnology, 41, .
(doi:10.1016/j.copbio.2016.05.001).
Abstract
Stable isotope probing of RNA has enthused researchers right from its first introduction in 2002. The concept of a labelling-based detection of process-targeted microbes independent of cellular replication or growth has allowed for a much more direct handle on functionally relevant microbiota than by labelling of other biomarkers. This has led to a widespread application of the technology, and breakthroughs in our understanding of carbon flow in natural microbiomes, autotrophic and heterotrophic physiologies, microbial food webs, host-microbe interactions and environmental biotechnology. Recent studies detecting labelled mRNA demonstrate that RNA-SIP is not limited to the analysis of rRNA, but is currently developing towards an approach for accessing targeted transcriptomes. In combination with next-generation sequencing and other methodological advances, RNA-SIP will continue to deliver invaluable insights into the functioning of microbial communities.
Text
COB_2016.docx
- Accepted Manuscript
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Accepted/In Press date: 5 May 2016
e-pub ahead of print date: 3 June 2016
Published date: October 2016
Organisations:
Centre for Biological Sciences, Environmental
Identifiers
Local EPrints ID: 396491
URI: http://eprints.soton.ac.uk/id/eprint/396491
ISSN: 0958-1669
PURE UUID: 6d58a3c7-bb22-48af-b849-82993012d5e7
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Date deposited: 10 Jun 2016 08:16
Last modified: 15 Mar 2024 05:38
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Contributors
Author:
Tillmann Lueders
Author:
Laura Bradford
Author:
Mike Manefield
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