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Diluted honey inhibits biofilm formation: potential application in urinary catheter management?

Diluted honey inhibits biofilm formation: potential application in urinary catheter management?
Diluted honey inhibits biofilm formation: potential application in urinary catheter management?
Aims Biofilms are ubiquitous and when mature have a complex structure of microcolonies in an extracellular polysaccharide and extracellular DNA matrix. Indwelling medical devices harbour biofilms which have been shown to cause infections and act as reservoirs for pathogens. Urinary catheters are often in place for considerable periods of time and are susceptible to both encrustation and biofilm formation. Strategies for minimising biofilm occurrence underpin an active research area in biomedicine. Manuka honey has, inter alia, well-established antibacterial properties. This study aims to assess the influence of honey on early biofilm formation in an established in vitro model. Methods An established model of early biofilm formation using static bacterial cultures in vinyl 96-well plates was used to grow Escherichia coli, strain ATC 25922 and Proteus mirabilis, strain 7002. Planktonic cells were removed and the residual biofilm was stained with crystal violet, which were subsequently eluted and quantified spectrophotometrically. Manuka honey (Unique Manuka Factor 15+) was added either with the bacteria or up to 72 hours after. Results Biofilms in this model was developed over 3 days, after which growth stalled. Mixed (1:1) cultures of E. coli and P. mirabilis grew slower than monocultures. In mixed cultures, honey gave a dose-dependent reduction in biofilm formation (between 3.3 and 16.7%w/v). At 72 hours, all concentrations inhibited maximally (p<0.001). Application of honey to cultures after 24 and 48 hours also reduced the adherent bacterial biomass (p<0.05–p<0.01). Conclusion Manuka honey at dilutions as low as 3.3% w/v in some protocols and at 10% or above in all protocols tested significantly inhibits bacterial attachment to a vinyl substrate and reduces further early biofilm development. No augmentation of growth over untreated controls was observed in any experiment.
0021-9746
140-144
Emineke, Somadina
aa12deec-d376-419f-bd82-9f694fc5e2e5
Cooper, Alan J.
65dcd1e1-3fcd-46b8-ad5f-f17e0d5b80a5
Fouch, Sarah
2684c009-ad17-4c80-ba6c-739eb8668510
Birch, Brian R.
536ee8d2-9cf9-4412-a29b-d2267fa9d765
Lwaleed, Bashir A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Emineke, Somadina
aa12deec-d376-419f-bd82-9f694fc5e2e5
Cooper, Alan J.
65dcd1e1-3fcd-46b8-ad5f-f17e0d5b80a5
Fouch, Sarah
2684c009-ad17-4c80-ba6c-739eb8668510
Birch, Brian R.
536ee8d2-9cf9-4412-a29b-d2267fa9d765
Lwaleed, Bashir A.
e7c59131-82ad-4a14-a227-7370e91e3f21

Emineke, Somadina, Cooper, Alan J., Fouch, Sarah, Birch, Brian R. and Lwaleed, Bashir A. (2017) Diluted honey inhibits biofilm formation: potential application in urinary catheter management? Journal of Clinical Pathology, 70 (2), 140-144. (doi:10.1136/jclinpath-2015-203546).

Record type: Article

Abstract

Aims Biofilms are ubiquitous and when mature have a complex structure of microcolonies in an extracellular polysaccharide and extracellular DNA matrix. Indwelling medical devices harbour biofilms which have been shown to cause infections and act as reservoirs for pathogens. Urinary catheters are often in place for considerable periods of time and are susceptible to both encrustation and biofilm formation. Strategies for minimising biofilm occurrence underpin an active research area in biomedicine. Manuka honey has, inter alia, well-established antibacterial properties. This study aims to assess the influence of honey on early biofilm formation in an established in vitro model. Methods An established model of early biofilm formation using static bacterial cultures in vinyl 96-well plates was used to grow Escherichia coli, strain ATC 25922 and Proteus mirabilis, strain 7002. Planktonic cells were removed and the residual biofilm was stained with crystal violet, which were subsequently eluted and quantified spectrophotometrically. Manuka honey (Unique Manuka Factor 15+) was added either with the bacteria or up to 72 hours after. Results Biofilms in this model was developed over 3 days, after which growth stalled. Mixed (1:1) cultures of E. coli and P. mirabilis grew slower than monocultures. In mixed cultures, honey gave a dose-dependent reduction in biofilm formation (between 3.3 and 16.7%w/v). At 72 hours, all concentrations inhibited maximally (p<0.001). Application of honey to cultures after 24 and 48 hours also reduced the adherent bacterial biomass (p<0.05–p<0.01). Conclusion Manuka honey at dilutions as low as 3.3% w/v in some protocols and at 10% or above in all protocols tested significantly inhibits bacterial attachment to a vinyl substrate and reduces further early biofilm development. No augmentation of growth over untreated controls was observed in any experiment.

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More information

Accepted/In Press date: 27 June 2016
e-pub ahead of print date: 26 September 2016
Published date: February 2017
Organisations: Faculty of Health Sciences

Identifiers

Local EPrints ID: 397631
URI: http://eprints.soton.ac.uk/id/eprint/397631
ISSN: 0021-9746
PURE UUID: 0546e426-5405-413e-a1c8-25f2d1817bee
ORCID for Bashir A. Lwaleed: ORCID iD orcid.org/0000-0001-5748-4892

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Date deposited: 04 Jul 2016 13:41
Last modified: 06 Aug 2024 01:39

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Contributors

Author: Somadina Emineke
Author: Alan J. Cooper
Author: Sarah Fouch
Author: Brian R. Birch

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