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Adaptive mechanisms and genomic plasticity for drought tolerance identified in European black poplar

Adaptive mechanisms and genomic plasticity for drought tolerance identified in European black poplar
Adaptive mechanisms and genomic plasticity for drought tolerance identified in European black poplar
Summer droughts are likely to increase in frequency and intensity across Europe, yet long-lived trees may have a limited ability to tolerate drought. It is therefore critical that we improve our understanding of phenotypic plasticity to drought in natural populations for ecologically and economically important trees such as Populus nigra L. A common garden experiment was conducted using ?500 wild P. nigra trees, collected from 11 river populations across Europe. Phenotypic variation was found across the collection, with southern genotypes from Spain and France characterized by small leaves and limited biomass production. To examine the relationship between phenotypic variation and drought tolerance, six genotypes with contrasting leaf morphologies were subjected to a water deficit experiment. ‘North eastern’ genotypes were collected at wet sites and responded to water deficit with reduced biomass growth, slow stomatal closure and reduced water use efficiency (WUE) assessed by ?13C. In contrast, ‘southern’ genotypes originating from arid sites showed rapid stomatal closure, improved WUE and limited leaf loss. Transcriptome analyses of a genotype from Spain (Sp2, originating from an arid site) and another from northern Italy (Ita, originating from a wet site) revealed dramatic differences in gene expression response to water deficit. Transcripts controlling leaf development and stomatal patterning, including SPCH, ANT, ER, AS1, AS2, PHB, CLV1, ERL1–3 and TMM, were down-regulated in Ita but not in Sp2 in response to drought.
0829-318X
909-928
Viger, Maud
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Smith, Hazel K.
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Cohen, David
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Dewoody, Jennifer
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Trewin, Harriet
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Steenackers, Marijke
cff8e155-e478-4bc7-bbc9-fca44a5ae9c8
Bastien, Catherine
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Taylor, Gail
f3851db9-d37c-4c36-8663-e5c2cb03e171
Viger, Maud
3f2de523-4a9f-44da-9f60-73d6cbc3edba
Smith, Hazel K.
9e5d9adc-6c4a-41a7-9418-e553275cd973
Cohen, David
ada227d5-129b-4db3-8a0d-0f72aa6b473b
Dewoody, Jennifer
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Trewin, Harriet
ca9a9ee7-dd07-4c57-8aa1-5fb4ec3cbbdc
Steenackers, Marijke
cff8e155-e478-4bc7-bbc9-fca44a5ae9c8
Bastien, Catherine
76976805-6404-4412-bff5-8c7c0ad6561f
Taylor, Gail
f3851db9-d37c-4c36-8663-e5c2cb03e171

Viger, Maud, Smith, Hazel K., Cohen, David, Dewoody, Jennifer, Trewin, Harriet, Steenackers, Marijke, Bastien, Catherine and Taylor, Gail (2016) Adaptive mechanisms and genomic plasticity for drought tolerance identified in European black poplar. Tree Physiology, 36 (7), 909-928. (doi:10.1093/treephys/tpw017).

Record type: Article

Abstract

Summer droughts are likely to increase in frequency and intensity across Europe, yet long-lived trees may have a limited ability to tolerate drought. It is therefore critical that we improve our understanding of phenotypic plasticity to drought in natural populations for ecologically and economically important trees such as Populus nigra L. A common garden experiment was conducted using ?500 wild P. nigra trees, collected from 11 river populations across Europe. Phenotypic variation was found across the collection, with southern genotypes from Spain and France characterized by small leaves and limited biomass production. To examine the relationship between phenotypic variation and drought tolerance, six genotypes with contrasting leaf morphologies were subjected to a water deficit experiment. ‘North eastern’ genotypes were collected at wet sites and responded to water deficit with reduced biomass growth, slow stomatal closure and reduced water use efficiency (WUE) assessed by ?13C. In contrast, ‘southern’ genotypes originating from arid sites showed rapid stomatal closure, improved WUE and limited leaf loss. Transcriptome analyses of a genotype from Spain (Sp2, originating from an arid site) and another from northern Italy (Ita, originating from a wet site) revealed dramatic differences in gene expression response to water deficit. Transcripts controlling leaf development and stomatal patterning, including SPCH, ANT, ER, AS1, AS2, PHB, CLV1, ERL1–3 and TMM, were down-regulated in Ita but not in Sp2 in response to drought.

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Accepted/In Press date: 14 February 2016
e-pub ahead of print date: 11 May 2016
Published date: 11 May 2016
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 397854
URI: http://eprints.soton.ac.uk/id/eprint/397854
ISSN: 0829-318X
PURE UUID: c05460cd-3037-49d8-8f5f-7e3e5db471b4
ORCID for Gail Taylor: ORCID iD orcid.org/0000-0001-8470-6390

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Date deposited: 08 Jul 2016 08:17
Last modified: 17 Dec 2019 06:44

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Contributors

Author: Maud Viger
Author: Hazel K. Smith
Author: David Cohen
Author: Jennifer Dewoody
Author: Harriet Trewin
Author: Marijke Steenackers
Author: Catherine Bastien
Author: Gail Taylor ORCID iD

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