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Nanoparticles and intracellular applications of surface-enhanced Raman spectroscopy

Nanoparticles and intracellular applications of surface-enhanced Raman spectroscopy
Nanoparticles and intracellular applications of surface-enhanced Raman spectroscopy
Surface-enhanced Raman spectroscopy (SERS) offers ultrasensitive vibrational fingerprinting at the nanoscale. Its non-destructive nature affords an ideal tool for interrogation of the intracellular environment, detecting the localisation of biomolecules, delivery and monitoring of therapeutics and for characterisation of complex cellular processes at the molecular level. Innovations in nanotechnology have produced a wide selection of novel, purpose-built plasmonic nanostructures capable of high SERS enhancement for intracellular probing while microfluidic technologies are being utilised to reproducibly synthesise nanoparticle (NP) probes at large scale and in high throughput. Sophisticated multivariate analysis techniques unlock the wealth of previously unattainable biomolecular information contained within large and multidimensional SERS datasets. Thus, with suitable combination of experimental techniques and analytics, SERS boasts enormous potential for cell based assays and to expand our understanding of the intracellular environment. In this review we trace the pathway to utilisation of nanomaterials for intracellular SERS. Thus we review and assess nanoparticle synthesis methods, their toxicity and cell interactions before presenting significant developments in intracellular SERS methodologies and how identified challenges can be addressed.
0003-2654
5037-5055
Taylor, Jack
42f9ee5f-1db7-4d14-8534-77078896004b
Huefner, Anna
2f24ff96-fe77-40a7-a06e-7813bbd10c86
Li, Li
8e6fbabd-aac6-4bb0-a273-a77750dc5b92
Wingfield, Jonathan
284e8cf4-f4fc-466c-86d2-410f7684e93e
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Taylor, Jack
42f9ee5f-1db7-4d14-8534-77078896004b
Huefner, Anna
2f24ff96-fe77-40a7-a06e-7813bbd10c86
Li, Li
8e6fbabd-aac6-4bb0-a273-a77750dc5b92
Wingfield, Jonathan
284e8cf4-f4fc-466c-86d2-410f7684e93e
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9

Taylor, Jack, Huefner, Anna, Li, Li, Wingfield, Jonathan and Mahajan, Sumeet (2016) Nanoparticles and intracellular applications of surface-enhanced Raman spectroscopy. Analyst, 141 (17), 5037-5055. (doi:10.1039/C6AN01003B).

Record type: Article

Abstract

Surface-enhanced Raman spectroscopy (SERS) offers ultrasensitive vibrational fingerprinting at the nanoscale. Its non-destructive nature affords an ideal tool for interrogation of the intracellular environment, detecting the localisation of biomolecules, delivery and monitoring of therapeutics and for characterisation of complex cellular processes at the molecular level. Innovations in nanotechnology have produced a wide selection of novel, purpose-built plasmonic nanostructures capable of high SERS enhancement for intracellular probing while microfluidic technologies are being utilised to reproducibly synthesise nanoparticle (NP) probes at large scale and in high throughput. Sophisticated multivariate analysis techniques unlock the wealth of previously unattainable biomolecular information contained within large and multidimensional SERS datasets. Thus, with suitable combination of experimental techniques and analytics, SERS boasts enormous potential for cell based assays and to expand our understanding of the intracellular environment. In this review we trace the pathway to utilisation of nanomaterials for intracellular SERS. Thus we review and assess nanoparticle synthesis methods, their toxicity and cell interactions before presenting significant developments in intracellular SERS methodologies and how identified challenges can be addressed.

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More information

Accepted/In Press date: 18 July 2016
e-pub ahead of print date: 19 July 2016
Published date: 7 September 2016
Organisations: Institute for Life Sciences, Chemical Biology Group

Identifiers

Local EPrints ID: 399449
URI: http://eprints.soton.ac.uk/id/eprint/399449
ISSN: 0003-2654
PURE UUID: 76096e09-331b-4bb4-8256-0d68b5340296
ORCID for Sumeet Mahajan: ORCID iD orcid.org/0000-0001-8923-6666

Catalogue record

Date deposited: 18 Aug 2016 07:46
Last modified: 18 Feb 2021 17:09

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