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Identification of active aerobic methanotrophs in plateau wetlands using DNA stable isotope probing

Identification of active aerobic methanotrophs in plateau wetlands using DNA stable isotope probing
Identification of active aerobic methanotrophs in plateau wetlands using DNA stable isotope probing
Sedge-dominated wetlands on the Qinghai–Tibetan Plateau are methane emission centers. Methanotrophs at these sites play a role in reducing methane emissions, but relatively little is known about the composition of active methanotrophs in these wetlands. Here, we used DNA stable isotope probing to identify the key active aerobic methanotrophs in three sedge-dominated wetlands on the plateau. We found that Methylocystis species were active in two peatlands, Hongyuan and Dangxiong. Methylobacter species were found to be active only in Dangxiong peat. Hongyuan peat had the highest methane oxidation rate, and cross-feeding of carbon from methanotrophs to methylotrophic Hyphomicrobium species was observed. Owing to a low methane oxidation rate during the incubation, the labeling of methanotrophs in Maduo wetland samples was not detected. Our results indicate that there are large differences in the activity of methanotrophs in the wetlands of this region.
0378-1097
1-8
Deng, Yongcui
5823c5fe-5a0d-4002-972c-48882272a423
Xiaoyong, Cui
253a2655-c75d-4059-8391-a012215e1e4b
Dumont, Marc G.
afd9f08f-bdbb-4cee-b792-1a7f000ee511
Deng, Yongcui
5823c5fe-5a0d-4002-972c-48882272a423
Xiaoyong, Cui
253a2655-c75d-4059-8391-a012215e1e4b
Dumont, Marc G.
afd9f08f-bdbb-4cee-b792-1a7f000ee511

Deng, Yongcui, Xiaoyong, Cui and Dumont, Marc G. (2016) Identification of active aerobic methanotrophs in plateau wetlands using DNA stable isotope probing. FEMS Microbiology Letters, 363 (16), 1-8. (doi:10.1093/femsle/fnw168). (PMID:27369086)

Record type: Article

Abstract

Sedge-dominated wetlands on the Qinghai–Tibetan Plateau are methane emission centers. Methanotrophs at these sites play a role in reducing methane emissions, but relatively little is known about the composition of active methanotrophs in these wetlands. Here, we used DNA stable isotope probing to identify the key active aerobic methanotrophs in three sedge-dominated wetlands on the plateau. We found that Methylocystis species were active in two peatlands, Hongyuan and Dangxiong. Methylobacter species were found to be active only in Dangxiong peat. Hongyuan peat had the highest methane oxidation rate, and cross-feeding of carbon from methanotrophs to methylotrophic Hyphomicrobium species was observed. Owing to a low methane oxidation rate during the incubation, the labeling of methanotrophs in Maduo wetland samples was not detected. Our results indicate that there are large differences in the activity of methanotrophs in the wetlands of this region.

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deng-et-al-FEMS-15th-June-Revision.docx - Accepted Manuscript
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Accepted/In Press date: 27 June 2016
e-pub ahead of print date: 1 July 2016
Organisations: Centre for Biological Sciences, Environmental

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Local EPrints ID: 399991
URI: http://eprints.soton.ac.uk/id/eprint/399991
ISSN: 0378-1097
PURE UUID: f8ac92e6-6f99-43e1-8a8d-66345f760d23
ORCID for Marc G. Dumont: ORCID iD orcid.org/0000-0002-7347-8668

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Date deposited: 06 Sep 2016 11:11
Last modified: 17 Dec 2019 06:39

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